Analysis of the subcellular trafficking properties of murine cytomegalovirus M78, a 7 transmembrane receptor homologue

Sharp, E. L., Davis-Poynter, N. J. and Farrell, H. E. (2009) Analysis of the subcellular trafficking properties of murine cytomegalovirus M78, a 7 transmembrane receptor homologue. Journal of General Virology, 90 1: 59-68.


Author Sharp, E. L.
Davis-Poynter, N. J.
Farrell, H. E.
Title Analysis of the subcellular trafficking properties of murine cytomegalovirus M78, a 7 transmembrane receptor homologue
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
1465-2099
Publication date 2009-01
Sub-type Article (original research)
DOI 10.1099/vir.0.004853-0
Volume 90
Issue 1
Start page 59
End page 68
Total pages 10
Editor R. M. Elliot
Place of publication Reading, Berks, U.K.
Publisher Society for General Microbiology
Collection year 2010
Language eng
Subject 0605 Microbiology
060502 Infectious Agents
060506 Virology
920109 Infectious Diseases
C1
Formatted abstract Murine cytomegalovirus (MCMV) M78 is a member of the betaherpesvirus ‘UL78 family’ of seven transmembrane receptor (7TMR) genes. Previous studies of M78 and its counterpart in rat cytomegalovirus (RCMV) have suggested that these genes are required for efficient cell–cell spread of their respective viruses in tissue culture and demonstrated that gene knockout viruses are significantly attenuated for replication in vivo. However, in comparison with other CMV 7TMRs, relatively little is known about the basic biochemical properties and subcellular trafficking of the UL78 family members. We have characterized MCMV M78 in both transiently transfected and MCMV-infected cells to determine whether M78 exhibits features in common with cellular 7TMR. We obtained preliminary evidence that M78 formed dimers, a property that has been reported for several cellular 7TMR. M78 traffics to the cell surface, but was rapidly and constitutively endocytosed. Antibody feeding experiments demonstrated co-localization of M78 with markers for both the clathrin-dependent and lipid raft/caveolae-mediated internalization pathways. In MCMV-infected cells, the subcellular localization of M78 was modified during the course of infection, which may be related to the incorporation of M78 into the virion envelope during the course of virion maturation.
Keyword Mouse cytomegalovirus
G protein-coupled receptor
Constitutive-endocytosis
Chemokine receptor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Thu, 03 Sep 2009, 09:05:57 EST by Mr Andrew Martlew on behalf of Clinical Medical Virology Centre