Regional expression of dopamine D1 and D2 receptor proteins in the cerebral cortex of asphyxic newborn infants

Kaewsuk, Sukit, Tannenberg, Rudi K., Kuo, Sheng-Wen, Bjorkman, S. Tracey, Govitrapong, Piyarat, Stadlin, Alfreda and Dodd, Peter R. (2009) Regional expression of dopamine D1 and D2 receptor proteins in the cerebral cortex of asphyxic newborn infants. Journal of Child Neurology, 24 2: 183-193. doi:10.1177/0883073808322669


Author Kaewsuk, Sukit
Tannenberg, Rudi K.
Kuo, Sheng-Wen
Bjorkman, S. Tracey
Govitrapong, Piyarat
Stadlin, Alfreda
Dodd, Peter R.
Title Regional expression of dopamine D1 and D2 receptor proteins in the cerebral cortex of asphyxic newborn infants
Formatted title
Regional expression of dopamine D1 and D2 receptor proteins in the cerebral cortex of asphyxic newborn infants
Journal name Journal of Child Neurology   Check publisher's open access policy
ISSN 0883-0738
1708-8283
Publication date 2009-02
Year available 2009
Sub-type Article (original research)
DOI 10.1177/0883073808322669
Volume 24
Issue 2
Start page 183
End page 193
Total pages 11
Editor Brumback, Roger
Place of publication United States
Publisher Sage Publications
Collection year 2010
Language eng
Subject C1
060110 Receptors and Membrane Biology
970106 Expanding Knowledge in the Biological Sciences
970111 Expanding Knowledge in the Medical and Health Sciences
Formatted abstract
Dopamine D1 and D2 receptor protein expression was examined by Western blotting in newborn infants dying from cerebral asphyxia between 31 and 42 weeks' gestation, and matched controls. Frontal, occipital, temporal, and motor cortex tissue samples were obtained at autopsy (median postmortem interval 35 hours) and frozen for storage at —80°C. A total of 2 immunoreactive bands were detected with each primary antibody in infant brain, whereas a single band was present in adult human and rat tissue. Immunoreactivity varied between cortical areas for both receptors, but their regional patterns differed significantly. D1 protein levels were higher in motor and temporal cortex than in frontal or occipital cortex. D2 protein showed graded expression frontal > motor > occipital > temporal cortex. Asphyxia cases showed lower expression of the upper D2 immunoreactive band, but no difference in regional pattern. Lower D2 receptor expression may attenuate stress responses and underlie increased vulnerability to hypoxia at birth. 
Copyright © 2009 by SAGE Publications
Keyword perinatal
autopsy
stress response
dopamine
receptor protein
asphyxia
ischemic brain injury
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Thu, 03 Sep 2009, 08:50:36 EST by Mr Andrew Martlew on behalf of School of Chemistry & Molecular Biosciences