Assessing risks of Wolbachia DNA cross-specimen contamination following mass collection and ethanol storage

Duplouy, A, Vermenot, C, Davies, N, Roderick, G, Hurst, GDD and Charlat, S (2009) Assessing risks of Wolbachia DNA cross-specimen contamination following mass collection and ethanol storage. MOLECULAR ECOLOGY RESOURCES, 9 1: 46-50. doi:10.1111/j.1755-0998.2008.02421.x


Author Duplouy, A
Vermenot, C
Davies, N
Roderick, G
Hurst, GDD
Charlat, S
Title Assessing risks of Wolbachia DNA cross-specimen contamination following mass collection and ethanol storage
Formatted title
Assessing risks of Wolbachia DNA cross-specimen contamination following mass collection and ethanol storage
Journal name MOLECULAR ECOLOGY RESOURCES   Check publisher's open access policy
ISSN 1755-098X
Publication date 2009-01
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1755-0998.2008.02421.x
Volume 9
Issue 1
Start page 46
End page 50
Total pages 5
Editor Livingstone, K.
Place of publication United Kingdom
Publisher Wiley- Blackwell Publishing
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
0603 Evolutionary Biology
Formatted abstract
Wolbachia and other intracellular bacteria that manipulate reproduction are widespread and can have major consequences on the ecology and evolution of their hosts. Several studies have attempted to assess the host range of these bacteria based on polymerase chain reaction assays on material preserved and collected using a variety of methods. While collecting in the field, mass storage in ethanol before sorting specimens in the laboratory is by far the easiest technique, and an integral component of Malaise trapping. This implicitly relies on the assumption that mass ethanol storage does not produce cross-contamination of Wolbachia DNA among specimens. Here we test this assumption. The absence of cross contamination between known positive and negative samples stored within a vial indicate there is no reason to believe collective storage of specimens creates artefactual increases in the incidence of Wolbachia or other intracellular bacteria.


Keyword Collection
contamination
ethanol storage
incidence
Wolbachia
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Thu, 03 Sep 2009, 08:48:58 EST by Mr Andrew Martlew on behalf of School of Biological Sciences