Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells

Zhang, Zhi-Yong, Teoh, Swee-Hin, Chong, Mark S.K, Schantz, Jan Thorsten, Fisk, Nicholas M., Choolani, Mahesh A. and Chan, Jerry (2009) Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells. Stem Cells, 27 1: 126-137. doi:10.1634/stemcells.2008-0456


Author Zhang, Zhi-Yong
Teoh, Swee-Hin
Chong, Mark S.K
Schantz, Jan Thorsten
Fisk, Nicholas M.
Choolani, Mahesh A.
Chan, Jerry
Title Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells
Journal name Stem Cells   Check publisher's open access policy
ISSN 1066-5099
Publication date 2009-01
Year available 2009
Sub-type Article (original research)
DOI 10.1634/stemcells.2008-0456
Volume 27
Issue 1
Start page 126
End page 137
Total pages 12
Place of publication Durham, NC, U.S.A
Publisher AlphaMed Press, Inc
Collection year 2010
Language eng
Subject C1
100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering)
Abstract Mesenchymal stem cells (MSCs) from human adult bone marrow (haMSCs) represent a promising source for bone tissue engineering. However, their low frequencies and limited proliferation restrict their clinical utility. Alternative postnatal, perinatal, and fetal sources of MSCs appear to have different osteogenic capacities, but have not been systematically compared with haMSCs. We investigated the proliferative and osteogenic potential of MSCs from human fetal bone marrow (hfMSCs), human umbilical cord (hUCMSCs), and human adult adipose tissue (hATMSCs), and haMSCs, both in monolayer cultures and after loading into three-dimensional polycaprolactone-tricalcium-phosphate scaffolds.Although all MSCs had comparable immunophenotypes, only hfMSCs and hUCMSCs were positive for the embryonic pluripotency markers Oct-4 and Nanog. hfMSCs expressed the lowest HLA-I level (55% versus 95%-99%) and the highest Stro-1 level (51% versus 10%-27%), and had the greatest colony-forming unit-fibroblast capacity (1.6x-2.0x; p < .01) and fastest doubling time (32 versus 54-111 hours; p < .01). hfMSCs had the greatest osteogenic capacity, as assessed by von-Kossa staining, alkaline phosphatase activity (5.1x-12.4x; p < .01), calcium deposition (1.6x-2.7x in monolayer and 1.6x-5.0x in scaffold culture; p < .01), calcium visualized on micro-computed tomography (3.9x17.6x; p < .01) and scanning electron microscopy, and osteogenic gene induction. Two months after implantation of cellular scaffolds in immunodeficient mice, hfMSCs resulted in the most robust mineralization (1.8x-13.3x; p < .01).The ontological and anatomical origins of MSCs have profound influences on the proliferative and osteogenic capacity of MSCs. hfMSCs had the most proliferative and osteogenic capacity of the MSC sources, as well as being the least immunogenic, suggesting they are superior candidates for bone tissue engineering.
Keyword Mesenchymal stem cells
Tissue engineering
Scaffold
Fetal
Umbilical cord
Adipose Tissue
bone marrow
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
2010 Higher Education Research Data Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 129 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 142 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 03 Sep 2009, 08:47:36 EST by Mr Andrew Martlew on behalf of UQ Centre for Clinical Research