A new PCR-based approach indicates the range of Clonorchis sinensis now extends to central Thailand

Traub, Rebecca J., Macaranas, Julie, Mungthin, Mathirut, Leelayoova, Saovanee, Cribb, Thomas, Murrell, K. Darwin and Thompson, R. C. Andrew (2009) A new PCR-based approach indicates the range of Clonorchis sinensis now extends to central Thailand. PLoS Neglected Tropical Diseases, 3 1: e367.1-e367.7. doi:10.1371/journal.pntd.0000367

Author Traub, Rebecca J.
Macaranas, Julie
Mungthin, Mathirut
Leelayoova, Saovanee
Cribb, Thomas
Murrell, K. Darwin
Thompson, R. C. Andrew
Title A new PCR-based approach indicates the range of Clonorchis sinensis now extends to central Thailand
Formatted title
A new PCR-based approach indicates the range of Clonorchis sinensis now extends to central Thailand
Journal name PLoS Neglected Tropical Diseases   Check publisher's open access policy
ISSN 1935-2727
Publication date 2009-01-20
Year available 2009
Sub-type Article (original research)
DOI 10.1371/journal.pntd.0000367
Open Access Status DOI
Volume 3
Issue 1
Start page e367.1
End page e367.7
Total pages 7
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2010
Language eng
Subject C1
110803 Medical Parasitology
E Expanding Knowledge
920404 Disease Distribution and Transmission (incl. Surveillance and Response)
Abstract Differentiation of the fish-borne trematodes belonging to the Opisthorchiidae, Heterophyidae and Lecithodendriidae is important from a clinical and epidemiological perspective, yet it is impossible to do using conventional coprological techniques, as the eggs are morphologically similar. Epidemiological investigation therefore currently relies on morphological examination of adult worms following expulsion chemotherapy. A PCR test capable of amplifying a segment of the internal transcribed spacer region of ribosomal DNA for the opisthorchiid and heterophyid flukes eggs taken directly from faeces was developed and evaluated in a rural community in central Thailand. The lowest quantity of DNA that could be amplified from individual adults of Opisthorchis viverrini, Clonorchis sinensis and Haplorchis taichui was estimated at 0.6 pg, 0.8 pg and 3 pg, respectively. The PCR was capable of detecting mixed infection with the aforementioned species of flukes under experimental conditions. A total of 11.6% of individuals in rural communities in Sanamchaikaet district, central Thailand, were positive for ‘Opisthorchis-like’ eggs in their faeces using conventional parasitological detection techniques. In comparison to microscopy, the PCR yielded a sensitivity and specificity of 71.0% and 76.7%, respectively. Analysis of the microscopy-positive PCR products revealed 64% and 23% of individuals to be infected with O. viverrini and C. sinensis, respectively. The remaining 13% (three individuals) were identified as eggs of Didymozoidae, presumably being passed mechanically in the faeces following the ingestion of infected fishes. An immediate finding of this study is the identification and first report of a C. sinensis–endemic community in central Thailand. This extends the known range of this liver fluke in Southeast Asia. The PCR developed herein provides an important tool for the specific identification of liver and intestinal fluke species for future epidemiological surveys.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article no. e367

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Veterinary Science Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 65 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 64 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 03 Sep 2009, 08:43:49 EST by Mr Andrew Martlew on behalf of School of Veterinary Science