The effect of platelet-rich plasma on osteoblast and periodontal ligament cell migration, proliferation and differentiation

Creeper, F, Lichanska, AM, Marshall, RI, Seymour, GJ and Ivanovski, S (2009) The effect of platelet-rich plasma on osteoblast and periodontal ligament cell migration, proliferation and differentiation. JOURNAL OF PERIODONTAL RESEARCH, 44 2: 258-265. doi:10.1111/j.1600-0765.2008.01125.x


Author Creeper, F
Lichanska, AM
Marshall, RI
Seymour, GJ
Ivanovski, S
Title The effect of platelet-rich plasma on osteoblast and periodontal ligament cell migration, proliferation and differentiation
Journal name JOURNAL OF PERIODONTAL RESEARCH   Check publisher's open access policy
ISSN 1600-0765
Publication date 2009-04
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1600-0765.2008.01125.x
Volume 44
Issue 2
Start page 258
End page 265
Total pages 8
Place of publication Malden MA, U.S.A.
Publisher Wiley - Blackwell Publishing, Inc.
Collection year 2010
Language eng
Subject C1
920113 Oro-Dental Disorders
110508 Periodontics
Abstract BACKGROUND AND OBJECTIVE: Platelet-rich plasma is used to deliver growth factors, in a safe and convenient manner, for enhancing bone and periodontal regeneration. However, conflicting reports regarding its effectiveness suggest that further study of the relevant cellular mechanisms is required. The aim of this study was to investigate the in vitro effect of platelet-rich plasma on osteoblasts and periodontal ligament cell function. MATERIAL AND METHODS: Various concentrations of platelet-rich plasma (100, 50, 20 and 10%) and platelet-poor plasma, obtained from human donors, were applied to primary cultures of human osteoblasts and periodontal ligament cells. [(3)H]-Thymidine incorporation, crystal violet staining and MTT assays were utilized to assess DNA synthesis and proliferation. Migration was determined by assessing the cell response to a concentration gradient, while differentiation was assessed using Alazarin Red staining. RESULTS: Platelet-rich plasma and platelet-poor plasma had stimulatory effects on the migration of both human osteoblasts and periodontal ligament cells. At 24 h, DNA synthesis was suppressed by the application of the various concentrations of platelet-rich plasma, but over a 5-d period, a beneficial effect on proliferation was observed, especially in response to 50% platelet-rich plasma. Platelet-poor plasma resulted in the greatest enhancement of cellular proliferation for both cell types. At a concentration of 50%, platelet-rich plasma and platelet-poor plasma facilitated differentiation of both cell types. CONCLUSION: Platelet-rich plasma can exert a positive effect on osteoblast and periodontal ligament cell function, but this effect is concentration specific with maximal concentrations not necessarily resulting in optimal outcomes. Platelet-poor plasma also appears to have the ability to promote wound healing-associated cell function.
Keyword platelet-rich plasma
GUIDED TISSUE REGENERATION
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Dentistry Publications
 
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Created: Thu, 03 Sep 2009, 08:40:30 EST by Mr Andrew Martlew on behalf of School of Dentistry