Cystathionine gamma-lyase is a component of cystine-mediated oxidative defense in Lactobacillus reuteri BR11

Lo, Raquel, Turner, Mark S., Barry, Daniel G., Sreekumar, Revathy, Walsh, Terence P. and Giffard, Philip M. (2009) Cystathionine gamma-lyase is a component of cystine-mediated oxidative defense in Lactobacillus reuteri BR11. Journal of Bacteriology, 191 6: 1827-1837. doi:10.1128/JB.01553-08

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Author Lo, Raquel
Turner, Mark S.
Barry, Daniel G.
Sreekumar, Revathy
Walsh, Terence P.
Giffard, Philip M.
Title Cystathionine gamma-lyase is a component of cystine-mediated oxidative defense in Lactobacillus reuteri BR11
Formatted title
Cystathionine γ-lyase is a component of cystine-mediated oxidative defense in Lactobacillus reuteri BR11
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
1098-5530
1067-8832
Publication date 2009-03
Sub-type Article (original research)
DOI 10.1128/JB.01553-08
Open Access Status File (Publisher version)
Volume 191
Issue 6
Start page 1827
End page 1837
Total pages 11
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2010
Language eng
Formatted abstract
Lactobacillus reuteri BR11 possesses a novel mechanism of oxidative defense involving an abundant cystine ABC transporter encoded by the cyuABC gene cluster. Large amounts of thiols, including H2S, are secreted upon cystine uptake by the CyuC transporter. A cystathionine γ-lyase (cgl) gene is cotranscribed with the cyu genes in several L. reuteri strains and was hypothesized to participate in cystine-mediated oxidative defense by producing reducing equivalents. This hypothesis was tested with L. reuteri BR11 by constructing a cgl mutant (PNG901) and comparing it to a similarly constructed cyuC mutant (PNG902). Although Cgl was required for H2S production from cystine, it was not crucial for oxidative defense in de Mann-Rogosa-Sharpe medium, in contrast to CyuC, whose inactivation resulted in lag-phase arrest in aerated cultures. The importance of Cgl in oxidative defense was seen only in the presence of hemin, which poses severe oxidative stress. The growth defects in aerated cultures of both mutants were alleviated by supplementation with cysteine (and cystine in the cgl mutant) but not methionine, with the cyuC mutant showing a much higher concentration requirement. We conclude that L. reuteri BR11 requires a high concentration of exogenous cysteine/cystine to grow optimally under aerobic conditions. This requirement is fulfilled by the abundant CyuC transporter, which has probably arisen due to the broad substrate specificity of Cgl, resulting in a futile pathway which degrades cystine taken up by the CyuC transporter to H2S. Cgl plays a secondary role in oxidative defense by its well-documented function of cysteine biosynthesis.
Keyword Lactic-acid bacteria
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Agriculture and Food Sciences
ERA 2012 Admin Only
 
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Created: Thu, 03 Sep 2009, 08:35:38 EST by Mr Andrew Martlew on behalf of School of Land, Crop and Food Sciences