Three-dimensional organization of fenestrae labyrinths in liver sinusoidal endothelial cells

Braet, Filip, Riches, James, Geerts, Willie, Jahn, Kristina A., Wisse, Eddie and Frederik, Peter (2009) Three-dimensional organization of fenestrae labyrinths in liver sinusoidal endothelial cells. Liver International, 29 4: 603-613. doi:10.1111/j.1478-3231.2008.01836.x


Author Braet, Filip
Riches, James
Geerts, Willie
Jahn, Kristina A.
Wisse, Eddie
Frederik, Peter
Title Three-dimensional organization of fenestrae labyrinths in liver sinusoidal endothelial cells
Journal name Liver International   Check publisher's open access policy
ISSN 1478-3223
1478-3231
Publication date 2009-04
Sub-type Article (original research)
DOI 10.1111/j.1478-3231.2008.01836.x
Volume 29
Issue 4
Start page 603
End page 613
Total pages 11
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Background/Aims: Liver sinusoidal endothelial cell (LSEC) fenestrae are membrane-bound pores that are grouped in sieve plates and act as a bidirectional guardian in regulating transendothelial liver transport. The high permeability of the endothelial lining is explained by the presence of fenestrae and by various membrane-bound transport vesicles. The question as to whether fenestrae relate to other transport compartments remains unclear and has been debated since their discovery almost 40 years ago.
Methods: In this study, novel insights concerning the three-dimensional (3D) organization of the fenestrated cytoplasm were built on transmission electron tomographical observations on isolated and cultured whole-mount LSECs. Classical transmission electron microscopy and atomic force microscopy imaging was performed to accumulate cross-correlative structural evidence.
Results and Conclusions: The data presented here indicate that different arrangements of fenestrae have to be considered: i.e. open fenestrae that lack any structural obstruction mainly located in the thin peripheral cytoplasm and complexes of multifolded fenestrae organized as labyrinth-like structures that are found in the proximity of the perinuclear area. Fenestrae in labyrinths constitute about one-third of the total LSEC porosity. The 3D reconstructions also revealed that coated pits and small membrane-bound vesicles are exclusively interspersed in the non-fenestrated cytoplasmic arms.
Keyword Atomic force microscopy
Electron tomography
Fenestrae labyrinth
Fenestrations
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Microscopy and Microanalysis Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 08:31:08 EST by Mr Andrew Martlew on behalf of Centre for Microscopy and Microanalysis