Evidence of heterogeneity in the antibody response against the platelet antigen 3a; recognition of an 11-mer peptide carrying the HPA-3a polymorphic determinant

de Leon, EJ, Yuan, FF, Pearson, H, Marquis, CP and Mahler, SM (2009) Evidence of heterogeneity in the antibody response against the platelet antigen 3a; recognition of an 11-mer peptide carrying the HPA-3a polymorphic determinant. VOX SANGUINIS, 96 3: 252-255. doi:10.1111/j.1423-0410.2008.01146.x


Author de Leon, EJ
Yuan, FF
Pearson, H
Marquis, CP
Mahler, SM
Title Evidence of heterogeneity in the antibody response against the platelet antigen 3a; recognition of an 11-mer peptide carrying the HPA-3a polymorphic determinant
Journal name VOX SANGUINIS   Check publisher's open access policy
ISSN 0042-9007
Publication date 2009-04
Year available 2009
Sub-type Article (original research)
DOI 10.1111/j.1423-0410.2008.01146.x
Volume 96
Issue 3
Start page 252
End page 255
Total pages 4
Editor Wolfgang R Mayr
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing Ltd.
Collection year 2010
Language eng
Subject C1
Abstract The immune processes involved in the development of alloantibodies against the human platelet antigens in alloimmune disorders remain unclear. Antibody recognition of the platelet antigens on their respective platelet glycoproteins has been shown to be dependent on glycoprotein conformation. Furthermore, the post-translational modification of glycoproteins adds complexity to the alloantigenic determinants. Nine anti-HPA-3a sera along with several control sera were tested for reactivity to an 11-mer peptide straddling the HPA-3a/b polymorphism. Sera found to specifically recognize the 3a peptide were further assessed by platelet pre-exposure and immunoblotting. Three of the nine antisera were found to specifically recognize an 11-mer synthetic 3a peptide by ELISA. Further analysis of all anti-HPA-3a sera by Western blot showed that only those reactive to the 3a peptide were able to bind both reduced and non-reduced GPIIb. The results presented in this study provide the first known evidence for the identification of an antibody population capable of recognizing a linear and non-glycosylated form of the HPA-3a epitope.
Keyword alloantibodies
GLYCOPROTEIN-IIIA
ALLOANTIGEN
LOCALIZATION
EXPRESSION
EPITOPES
DOMAIN
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Thu, 03 Sep 2009, 08:30:37 EST by Mr Andrew Martlew on behalf of Aust Institute for Bioengineering & Nanotechnology