Annexin A2-dependent polymerization of actin mediates endosome biogenesis

Morel, E, Parton, R. G. and Gruenberg, J (2009) Annexin A2-dependent polymerization of actin mediates endosome biogenesis. Developmental Cell, 16 3: 445-457. doi:10.1016/j.devcel.2009.01.007

Author Morel, E
Parton, R. G.
Gruenberg, J
Title Annexin A2-dependent polymerization of actin mediates endosome biogenesis
Journal name Developmental Cell   Check publisher's open access policy
ISSN 1534-5807
Publication date 2009-03
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.devcel.2009.01.007
Volume 16
Issue 3
Start page 445
End page 457
Total pages 13
Editor Deborah Sweet
Place of publication Cambridge, MA, U.S.A.
Publisher Cell Press
Collection year 2010
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060199 Biochemistry and Cell Biology not elsewhere classified
Abstract Early endosomes give rise to multivesicular intermediates during transport toward late endosomes. Much progress has been made in understanding the sorting of receptors into these intermediates, but the mechanisms responsible for their biogenesis remain unclear. Here, we report that F-actin is necessary for transport beyond early endosomes and endosome formation. We found that endosomes captured by actin cables were essentially stationary, but early endosomes also exhibited patches of F-actin and facilitated selective F-actin nucleation and polymerization. Our data show that nucleation of actin patches by early endosomes is strictly dependent on annexin A2, a protein involved in early-to-late endosome transport. It also requires the actin nucleation factor Spire1 and involves Arp2/3, which is needed for filament branching. We conclude that actin patches are nucleated on early endosomes via annexin A2 and Spire1, and that these patches control endosome biogenesis, presumably by driving the membrane remodeling process.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 89 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 08:29:51 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience