Real-time polymerase chain reaction (PCR) assays for the specific detection and quantification of seven Eimeria species that cause coccidiosis in chickens

Morgan, J. A. T., Morris, G. M., Wlodek, B. M., Byrnes, R., Jenner, M., Constantinoiu, C. C, Anderson, G. R., Lew-Tabor, A. E., Molloy, J. B., Gasser, R. B. and Jorgensen, W. K. (2009) Real-time polymerase chain reaction (PCR) assays for the specific detection and quantification of seven Eimeria species that cause coccidiosis in chickens. Molecular and Cellular Probes, 23 2: 83-89. doi:10.1016/j.mcp.2008.12.005


Author Morgan, J. A. T.
Morris, G. M.
Wlodek, B. M.
Byrnes, R.
Jenner, M.
Constantinoiu, C. C
Anderson, G. R.
Lew-Tabor, A. E.
Molloy, J. B.
Gasser, R. B.
Jorgensen, W. K.
Title Real-time polymerase chain reaction (PCR) assays for the specific detection and quantification of seven Eimeria species that cause coccidiosis in chickens
Formatted title
Real-time polymerase chain reaction (PCR) assays for the specific detection and quantification of seven Eimeria species that cause coccidiosis in chickens
Journal name Molecular and Cellular Probes   Check publisher's open access policy
ISSN 0890-8508
1096-1194
Publication date 2009-04
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.mcp.2008.12.005
Volume 23
Issue 2
Start page 83
End page 89
Total pages 7
Place of publication London, United Kingdom
Publisher Academic Press
Collection year 2010
Language eng
Formatted abstract
Coccidiosis of chickens is an economically important disease caused by infection with species of Eimeria. The oocysts of some of the seven recognized species are difficult to distinguish morphologically and for this reason diagnostic laboratories are increasingly utilizing DNA-based technologies for the specific identification of Eimeria. The real-time PCR provides both sensitivity and speed for the analysis of DNA samples, and the approach has the capability of quantifying DNA. Together with a protocol for the extraction of DNA directly from faecal samples, real-time PCR assays have been established for the detection and quantification of seven species of Eimeria that infect chickens in Australia. The assays target one genetic marker, the second internal transcribed spacer of nuclear ribosomal DNA (ITS-2), use TaqMan® MGB technology with species-specific probes, and can be multiplexed in pairs such that the seven species of Eimeria can be screened in four reaction tubes. A test screen of commercial flocks identified more Eimeria-infected chickens than were detected by coproscopic examination for oocysts. These molecular assays can also be used for the quality control of mixed-species vaccines. The ability to multiplex the assays makes them particularly practical for screening samples from chickens with mixed-species infections where the relative abundance of each Eimeria species present is required.
Keyword Coccidiosis
Eimeria
Diagnosis
Real-time PCR
Nuclear ribosomal DNA
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Available online 27 December 2008

 
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Created: Thu, 03 Sep 2009, 08:28:35 EST by Mr Andrew Martlew on behalf of School of Veterinary Science