The cell cycle of the planctomycete Gemmata obscuriglobus with respect to cell compartmentalization

Lee, Kuo-Chang, Webb, Rick I. and Fuerst, John A. (2009) The cell cycle of the planctomycete Gemmata obscuriglobus with respect to cell compartmentalization. BMC Cell Biology, 10 4: 1-12. doi:10.1186/1471-2121-10-4


Author Lee, Kuo-Chang
Webb, Rick I.
Fuerst, John A.
Title The cell cycle of the planctomycete Gemmata obscuriglobus with respect to cell compartmentalization
Formatted title
The cell cycle of the planctomycete Gemmata obscuriglobus with respect to cell compartmentalization
Journal name BMC Cell Biology   Check publisher's open access policy
ISSN 1471-2121
Publication date 2009-01-14
Sub-type Article (original research)
DOI 10.1186/1471-2121-10-4
Open Access Status DOI
Volume 10
Issue 4
Start page 1
End page 12
Total pages 12
Editor Dr. Melissa Norton
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2010
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060402 Cell and Nuclear Division
060501 Bacteriology
Formatted abstract
Background:  Gemmata obscuriglobus  is a distinctive member of the divergent phylum Planctomycetes, all known members of which are peptidoglycan-less bacteria with a shared compartmentalized cell structure and divide by a budding process.  G. obscuriglobus  in addition shares the unique feature that its nucleoid DNA is surrounded by an envelope consisting of two membranes forming an analogous structure to the membrane-bounded nucleoid of eukaryotes and therefore  G. obscuriglobus  forms a special model for cell biology. Draft genome data for  G. obscuriglobus  as well as complete genome sequences  available so far for other planctomycetes indicate that the key bacterial cell division protein FtsZ is not present in these planctomycetes, so the cell division process in planctomycetes is  of special comparative interest. The membranebounded nature of the nucleoid in G. obscuriglobus also suggests that special mechanisms for the distribution of this nuclear body to the bud and for distribution of chromosomal DNA might exist during division. It was therefore of interest to examine the cell division cycle in G. obscuriglobus and the process of nucleoid distribution and nuclear  body  formation  during  division  in  this planctomycete bacterium via light and electron microscopy.

Results:  Using phase contrast and fluorescence light microscopy, and transmission electron microscopy, the cell division cycle of G. obscuriglobus was determined. During the budding process, the bud was formed and developed  in size from one point of the mother cell perimeter until separation. The matured daughter cell acted as a new mother cell and started its own budding cycle while the mother cell can itself initiate budding repeatedly. Fluorescence microscopy of DAPIstained cells of G. obscuriglobus suggested that translocation of the nucleoid and formation of the bud  did not occur at the same time. Confocal laser scanning light microscopy applied to cells stained for membranes as well  as DNA confirmed the behaviour of the nucleoid and nucleoid envelope during cell division. Electron microscopy of cryosubstituted cells confirmed deductions from light microscopy concerning nucleoid presence in relation  to the stage of budding, and showed that the nucleoid was observed to occur in both mother and bud cells only at later budding stages. It further suggested that nucleoid envelope formed only after the nucleoid was translocated into the bud, since envelopes only appeared in more mature buds, while naked nucleoids occurred in smaller buds. Nucleoid envelope appeared to originate from the intracytoplasmic membranes (ICM) of both mother cell and bud. There was always a connecting passage between mother cell and bud during the budding process until separation of the two cells. The division cycle of the nucleated planctomycete G. obscuriglobus appears to be a complex process in which chromosomal DNA is transported to the daughter cell bud after initial formation of the bud, and this can be performed repeatedly by a single mother cell. 

Conclusion: The division cycle of the nucleated planctomycete G. obscuriglobus is a complex process in which chromosomal nucleoid DNA is transported to the daughter cell bud after initial formation of a bud without nucleoid. The new bud nucleoid is initially naked and not surrounded by membrane, but eventually acquires a complete nucleoid envelope consisting of two closely apposed membranes as occurs in the mother cell. The membranes of the new nucleoid envelope surrounding the bud nucleoid are derived from intracytoplasmic membranes of both the mother cell and the bud. The cell division of G. obscuriglobus displays some unique features not known in cells of either prokaryotes or eukaryotes.   
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Thu, 03 Sep 2009, 08:22:51 EST by Mr Andrew Martlew on behalf of School of Chemistry & Molecular Biosciences