Genomic expression profiling of mature soybean (Glycine max) pollen

Haerizadeh, Farzad, Wong, Chui E., Bhalla, PL, Gresshoff, Peter M. and Singh, Mohan B. (2009) Genomic expression profiling of mature soybean (Glycine max) pollen. BMC Plant Biology, 9 Article# 25: 25-1-25-12. doi:10.1186/1471-2229-9-25

Author Haerizadeh, Farzad
Wong, Chui E.
Bhalla, PL
Gresshoff, Peter M.
Singh, Mohan B.
Title Genomic expression profiling of mature soybean (Glycine max) pollen
Formatted title
Genomic expression profiling of mature soybean (Glycine max) pollen
Journal name BMC Plant Biology   Check publisher's open access policy
ISSN 1471-2229
Publication date 2009-03
Year available 2009
Sub-type Article (original research)
DOI 10.1186/1471-2229-9-25
Open Access Status DOI
Volume 9
Issue Article# 25
Start page 25-1
End page 25-12
Total pages 12
Editor Eva-Mari Aro
Place of publication United Kingdom
Publisher BioMed Central
Collection year 2010
Language eng
Subject C1
820503 Grain Legumes
820405 Soybeans
829899 Environmentally Sustainable Plant Production not elsewhere classified
060702 Plant Cell and Molecular Biology
060408 Genomics
060102 Bioinformatics
Formatted abstract
Background: Pollen, the male partner in the reproduction of flowering plants, comprises either two or three cells at maturity. The current knowledge of the pollen transcriptome is limited to the model plant systems Arabidopsis thaliana and Oryza sativa which have tri-cellular pollen grains at maturity. Comparative studies on pollen of other genera, particularly crop plants, are needed to understand the pollen gene networks that are subject to functional and evolutionary conservation.  In this study, we used the Affymetrix Soybean GeneChip® to perform transcriptional profiling on
mature bi-cellular soybean pollen.
Results: Compared to the sporophyte transcriptome, the soybean pollen transcriptome revealed a restricted and unique repertoire of genes, with a significantly greater proportion of specifically expressed genes than is found in the sporophyte tissue. Comparative analysis shows that, among the 37,500 soybean transcripts addressed in this study, 10,299 transcripts (27.46%) are expressed in pollen. Of the pollen-expressed sequences, about 9,489 (92.13%) are also expressed in
sporophytic tissues, and 810 (7.87%) are selectively expressed in pollen. Overall, the soybean pollen transcriptome shows an enrichment of transcription factors (mostly zinc finger family proteins), signal recognition receptors, transporters, heat shock-related proteins and members of the ubiquitin proteasome proteolytic pathway.
Conclusion: This is the first report of a soybean pollen transcriptional profile. These data extend our current knowledge regarding regulatory pathways that govern the gene regulation and development of pollen. A comparison between transcription factors up-regulated in soybean and those in Arabidopsis revealed some divergence in the numbers and kinds of regulatory proteins expressed in both species.
Keyword Heat-shock protein
Male gametophyte development
Transcription factor family
Calmodulin-binding protein
Tube growth
Monosaccharide transporter
Gene expression
Finger protein
DNA binding
Root growth
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
2010 Higher Education Research Data Collection
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Citation counts: TR Web of Science Citation Count  Cited 42 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 40 times in Scopus Article | Citations
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Created: Thu, 03 Sep 2009, 08:22:38 EST by Mr Andrew Martlew on behalf of Centre for Integrative Legume Research