Isoprene synthesis protects transgenic tobacco plants from oxidative stress

Vickers, Claudia E., Possell, Malcolm, Cojocariu, Cristian I., Velikova, Violeta B., Laothawornkitkul, Jullada, Ryan, Annette, Mullineaux, Philip M. and Hewitt, C. Nicholas (2009) Isoprene synthesis protects transgenic tobacco plants from oxidative stress. Plant, Cell and Environment, 32 5: 520-531. doi:10.1111/j.1365-3040.2009.01946.x

Author Vickers, Claudia E.
Possell, Malcolm
Cojocariu, Cristian I.
Velikova, Violeta B.
Laothawornkitkul, Jullada
Ryan, Annette
Mullineaux, Philip M.
Hewitt, C. Nicholas
Title Isoprene synthesis protects transgenic tobacco plants from oxidative stress
Journal name Plant, Cell and Environment   Check publisher's open access policy
ISSN 0140-7791
Publication date 2009-05
Sub-type Article (original research)
DOI 10.1111/j.1365-3040.2009.01946.x
Volume 32
Issue 5
Start page 520
End page 531
Total pages 12
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Subject 0607 Plant Biology
Abstract Isoprene emission represents a significant loss of carbon to those plant species that synthesize this highly volatile and reactive compound. As a tool for studying the role of isoprene in plant physiology and biochemistry, we developed transgenic tobacco plants capable of emitting isoprene in a similar manner to and at rates comparable to a naturally emitting species. Thermotolerance of photosynthesis against transient high-temperature episodes could only be observed in lines emitting high levels of isoprene; the effect was very mild and could only be identified over repetitive stress events. However, isoprene-emitting plants were highly resistant to ozone-induced oxidative damage compared with their non-emitting azygous controls. In ozone-treated plants, accumulation of toxic reactive oxygen species (ROS) was inhibited, and antioxidant levels were higher. Isoprene-emitting plants showed remarkably decreased foliar damage and higher rates of photosynthesis compared to non-emitting plants immediately following oxidative stress events. An inhibition of hydrogen peroxide accumulation in isoprene-emitting plants may stall the programmed cell death response which would otherwise lead to foliar necrosis. These results demonstrate that endogenously produced isoprene provides protection from oxidative damage.
Keyword isoprene synthase
oxidative damage
reactive oxygen species
Q-Index Code C1
Q-Index Status Provisional Code

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Thu, 03 Sep 2009, 08:20:01 EST by Mr Andrew Martlew on behalf of Aust Institute for Bioengineering & Nanotechnology