Incubation temperature critical to successful stimulation of in vitro zygotic embryo growth in four Australian native Cyperaceae species

Panaia, M., Bunn, E., Turner, S.R. and McComb, J. (2009) Incubation temperature critical to successful stimulation of in vitro zygotic embryo growth in four Australian native Cyperaceae species. Plant Cell Tissue And Organ Culture, 97 2: 197-202. doi:10.1007/s11240-009-9515-0


Author Panaia, M.
Bunn, E.
Turner, S.R.
McComb, J.
Title Incubation temperature critical to successful stimulation of in vitro zygotic embryo growth in four Australian native Cyperaceae species
Journal name Plant Cell Tissue And Organ Culture   Check publisher's open access policy
ISSN 0167-6857
Publication date 2009-05
Sub-type Article (original research)
DOI 10.1007/s11240-009-9515-0
Volume 97
Issue 2
Start page 197
End page 202
Total pages 6
Place of publication Netherlands
Publisher Springer Netherlands
Language eng
Abstract Many species of Western Australian Cyperaceae (sedges) are vital components of the indigenous flora but commonly display low seed set, poor seed quality and intractable seed dormancy. We report the effects of incubation temperature and in vitro growth media on whole seed germination compared with extracted zygotic embryo growth in Tetraria capillaris, T. octandra, Lepidosperma drummondii and L. tenue. No germination was observed from intact whole seeds of all test species regardless of the treatment evaluated. In contrast, excised zygotic embryos of all study species exhibited significant increases in growth when cultured at 15°C compared to embryos incubated at 25°C; however, optimal media for embryo growth were genera specific. Extracted embryos of T. capillaris and T. octandra exhibited maximum percentage growth (30 and 40%, respectively) at 15°C on 1/2 MS medium with no plant growth regulators required. In the case of L. drummondii and L. tenue 1 μM thidiazuron was a necessary addition to the 1/2 MS medium resulting in 40 and 77% growth of embryos (at 15°C), respectively. Incubation of extracted embryos at 25°C (regardless of medium treatment) resulted in <10% embryo growth for T. octandra and L. tenue, while the remaining two species (L. drummondii, T. capillaris) showed no embryo growth at 25°C on any medium treatment.
Keyword Lepidosperma drummondii
Lepidosperma tenue
Temperature
Tetraria capillaris
Tetraria octandra
Seed germination
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Thu, 03 Sep 2009, 08:19:58 EST by Mr Andrew Martlew on behalf of School of Pharmacy