Low rates of Pseudomonas aeruginosa misidentification in isolates from Cystic Fibrosis patients.

Kidd, Timothy J., Ramsay, Kay A., Hu, Honghua Hu, Bye, Peter T. P., Elkins, Mark R., Grimwood, Keith, Harbour, Colin, Marks, Guy B., Nissen, Michael D., Robinson, Phillip J., Rose, Barbara R., Sloots, Theo P., Wainwright, Claire E., Bell, Scott C. and ACPinCF Investigators (2009) Low rates of Pseudomonas aeruginosa misidentification in isolates from Cystic Fibrosis patients.. Journal of Clinical Microbiology, 47 5: 1503-1509. doi:10.1128/JCM.00014-09

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Author Kidd, Timothy J.
Ramsay, Kay A.
Hu, Honghua Hu
Bye, Peter T. P.
Elkins, Mark R.
Grimwood, Keith
Harbour, Colin
Marks, Guy B.
Nissen, Michael D.
Robinson, Phillip J.
Rose, Barbara R.
Sloots, Theo P.
Wainwright, Claire E.
Bell, Scott C.
ACPinCF Investigators
Title Low rates of Pseudomonas aeruginosa misidentification in isolates from Cystic Fibrosis patients.
Formatted title
Low rates of Pseudomonas aeruginosa misidentification in isolates from Cystic Fibrosis patients.
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
Publication date 2009-05
Year available 2009
Sub-type Article (original research)
DOI 10.1128/JCM.00014-09
Open Access Status File (Publisher version)
Volume 47
Issue 5
Start page 1503
End page 1509
Total pages 7
Editor Andrew B Orderdonk
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2010
Language eng
Formatted abstract
´╗┐Pseudomonas aeruginosa is an important cause of pulmonary infection in cystic fibrosis (CF). Its correct identification ensures elfective patient management and infection control strategies. However, little is known about how often CF sputum isolates are falsely identified as P. aeruginosa. We used P. aerugillosa-specific duplex real-time PCR assays to determine if 2,267 P. aeruginosa sputum isolates from 561 CF patients were correctly identified by 17 Australian clinical microbiology laboratories. Misidentified isolates underwent further phenotypic tests, amplified rRNA gene restriction analysis, and partial 168 rRNA gene sequence analysis. Participating laboratories were surveyed on how they identified P. aeruginosa from CF sputum. Overall, 2,214 (97.7%) isolates from 531 (94.7%) CF patients were correctly identified as P. aeruginosa. Further testing with the API 20NE kit correctly identified only 34 (59%) of the misidentified isolates. Twelve (40%) patients had previously grown the misidentified species in their sputum. Achromobacter xylosoxidans (11 = 21), Stenotrophomonas maltophilia (11 = 15), and Inquilinus limosus in = 4) were the species most commonly misidentified as P. aeruginosa. Overall, there were very low ra.tes of P. aeruginosa misidentification among isolates from a broad cross section of Australian CF patients. Additional improvements are possible by undertaking a culture history review, noting colonial morphology, and performing stringent oxidase, DNase, and colistin susceptibility testing for all presumptive P. aeruginosa isolates. Isolates exhibiting atypical phenotypic features should be evaluated further by additional phenotypic or genotypic identification techniques.

Keyword Pseudomonas aeruginosa
Ribosomal-RNA gene
Cystic Fibrosis
molecular identification
Q-Index Code C1
Q-Index Status Confirmed Code

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Created: Thu, 03 Sep 2009, 08:13:32 EST by Mr Andrew Martlew on behalf of Clinical Medical Virology Centre