Phylogenetic and molecular analysis of hydrogen-producing green algae

Timmins, Matthew, Thomas-Hall, Skye R., Darling, Aaron, Zhang, Eugene, Hankamer, Ben, Marx, Ute C and Schenk, Peer M. (2009) Phylogenetic and molecular analysis of hydrogen-producing green algae. Journal of Experimental Botany, 60 6: 1691-1702. doi:10.1093/jxb/erp052


Author Timmins, Matthew
Thomas-Hall, Skye R.
Darling, Aaron
Zhang, Eugene
Hankamer, Ben
Marx, Ute C
Schenk, Peer M.
Title Phylogenetic and molecular analysis of hydrogen-producing green algae
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 0022-0957
1460-2431
Publication date 2009-04
Year available 2009
Sub-type Article (original research)
DOI 10.1093/jxb/erp052
Volume 60
Issue 6
Start page 1691
End page 1702
Total pages 12
Editor David Prosser
Place of publication Oxford, U. K.
Publisher Oxford University Press
Collection year 2010
Language eng
Subject C1
850303 Hydrogen Production from Renewable Energy
850309 Production of Biofuels (Biomass)
060705 Plant Physiology
Formatted abstract
A select set of microalgae are reported to be able to catalyse photobiological H2 production from water. Based on the model organism Chlamydomonas reinhardtii, a method was developed for the screening of naturally occurring H2-producing microalgae. By purging algal cultures with N2 in the dark and subsequent illumination, it is possible to rapidly induce photobiological H2 evolution. Using NMR spectroscopy for metabolic profiling in C. reinhardtii, acetate, formate, and ethanol were found to be key compounds contributing to metabolic variance during the assay. This procedure can be used to test algal species existing as axenic or mixed cultures for their ability to produce H2. Using this system, five algal isolates capable of H2 production were identified in various aquatic systems. A phylogenetic tree was constructed using ribosomal sequence data of green unicellular algae to determine if there were taxonomic patterns of H2 production. H2-producing algal species were seen to be dispersed amongst most clades, indicating an H2-producing capacity preceded evolution of the phylum Chlorophyta.
Keyword Biohydrogen
Photobiological H2 production
Chlorophyta
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Thu, 03 Sep 2009, 08:13:22 EST by Mr Andrew Martlew on behalf of School of Biological Sciences