Effect of exogenous nitric oxide on murine splenic immune response induced by Aggregatibacter actinomycetemcomitans lipopolysaccharide

Sosroseno, W, Bird, PS and Seymour, GJ (2009) Effect of exogenous nitric oxide on murine splenic immune response induced by Aggregatibacter actinomycetemcomitans lipopolysaccharide. ANAEROBE, 15 3: 95-98. doi:10.1016/j.anaerobe.2009.01.002


Author Sosroseno, W
Bird, PS
Seymour, GJ
Title Effect of exogenous nitric oxide on murine splenic immune response induced by Aggregatibacter actinomycetemcomitans lipopolysaccharide
Formatted title
Effect of exogenous nitric oxide on murine splenic immune response induced by Aggregatibacter actinomycetemcomitans lipopolysaccharide
Journal name ANAEROBE   Check publisher's open access policy
ISSN 1075-9964
Publication date 2009-06
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.anaerobe.2009.01.002
Volume 15
Issue 3
Start page 95
End page 98
Total pages 4
Place of publication Oxford , U.K
Publisher Elsevier Sci Ltd
Collection year 2010
Language eng
Subject C1
920113 Oro-Dental Disorders
110508 Periodontics
Abstract The aim of this study was to determine the effect of exogenous nitric oxide (NO) on the induction of murine splenic immune response to Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS) in vitro. BALB/c mice were immunized with A. actinomycetemcomitans LPS and a control group was sham-immunized. Spleen cells were obtained, cultured and stimulated with A. actinomycetemcomitans LPS with or without the presence of S-nitroso acetyl-penicillamine (SNAP), a NO donor, and carboxy-PTIO, an NO scavenger. Culture supernatants were assessed for inducible nitric oxide synthase (iNOS) activity, specific IgG subclass levels, and both IFN-gamma and IL-4 levels. The results showed that in A. actinomycetemcomitans LPS-stimulated cells, SNAP enhances iNOS activity but inhibits the levels of specific IgG2a and IFN-gamma suggesting a Th1 response. The effect of SNAP on these immune parameters was ablated by carboxy-PTIO. These results suggest that exogenous NO may suppress the Th1-like immune response of A. actinomycetemcomitans LPS-stimulated murine spleen cells.
Keyword A. actinomycetemcomitans
ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Dentistry Publications
 
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Created: Thu, 03 Sep 2009, 08:07:51 EST by Mr Andrew Martlew on behalf of School of Dentistry