Haplotype analyses in wheat for complex traits: Tracking the chromosome 3B and 7B regions associated with late maturity alpha amylase (LMA) in breeding programs

McNeil, M. D., Diepeveen, D., Wilson, R., Barclay, I., McLean, R., Chalhoub, B. and Appels, R. (2009) Haplotype analyses in wheat for complex traits: Tracking the chromosome 3B and 7B regions associated with late maturity alpha amylase (LMA) in breeding programs. Crop and Pasture Science, 60 5: 463-471.


Author McNeil, M. D.
Diepeveen, D.
Wilson, R.
Barclay, I.
McLean, R.
Chalhoub, B.
Appels, R.
Title Haplotype analyses in wheat for complex traits: Tracking the chromosome 3B and 7B regions associated with late maturity alpha amylase (LMA) in breeding programs
Journal name Crop and Pasture Science   Check publisher's open access policy
ISSN 1836-5795
1836-5795
Publication date 2009
Sub-type Article (original research)
DOI 10.1071/CP08340
Volume 60
Issue 5
Start page 463
End page 471
Total pages 9
Place of publication Collingwood, Vic., Australia
Publisher CSIRO Publishing
Language eng
Abstract The quantitative trait loci (QTLs) on chromosomes 7BL and 3BS from Halberd have been used as a major source of tolerance to late maturity α amylase (LMA) within Australian wheat breeding programs. New simple sequence repeat (SSR) markers identified from the sequencing of Bacterial Artificial Chromosome (BAC) clones from the wheat cv. Renan library, and known SSRs, were used to characterise these major QTLs. The reduction or elimination of the LMA defect in wheat cultivars is a major goal for wheat breeding programs and is confounded by the complexity in measuring the trait unambiguously. In this haplotyping study focussing on two significant chromosomal regions, markers and combinations of markers were investigated for their ability to discriminate between 39 Australian and Mexican wheat lines differing in levels of LMA. Genetic relationships among these wheat lines estimated by cluster analysis of molecular marker data were combined with phenotypic information in order to calibrate the genotypes of the wheat lines against their LMA phenotype. It was evident that some SSRs from the respective QTLs had greater discriminating power than others to identify LMA phenotypes. Discrimination was not, however, absolute and a statistical analysis of the data defined a risk factor associated with particular combinations of alleles, for use in early selection or backcrossing.
Keyword Haplotype diversity
Microsatellite markers
Bacterial Artificial Chromosome
SSR
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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Created: Thu, 03 Sep 2009, 08:07:16 EST by Mr Andrew Martlew on behalf of Qld Alliance for Agriculture and Food Innovation