A Novel ABA Insensitive Mutant of Lotus japonicus with a Wilty Phenotype Displays Unaltered Nodulation Regulation

Biswas, B, Chan, PK and Gresshoff, PM (2009) A Novel ABA Insensitive Mutant of Lotus japonicus with a Wilty Phenotype Displays Unaltered Nodulation Regulation. Molecular Plant, 2 3: 487-499. doi:10.1093/mp/ssp009


Author Biswas, B
Chan, PK
Gresshoff, PM
Title A Novel ABA Insensitive Mutant of Lotus japonicus with a Wilty Phenotype Displays Unaltered Nodulation Regulation
Formatted title
A Novel ABA Insensitive Mutant of Lotus japonicus with a Wilty Phenotype Displays Unaltered Nodulation Regulation
Journal name Molecular Plant   Check publisher's open access policy
ISSN 1752-9867
1674-2052
Publication date 2009-05
Year available 2009
Sub-type Article (original research)
DOI 10.1093/mp/ssp009
Volume 2
Issue 3
Start page 487
End page 499
Total pages 13
Editor Sheng Luan
Place of publication United Kingdom
Publisher Oxford University Press
Collection year 2010
Language eng
Subject C1
820405 Soybeans
829899 Environmentally Sustainable Plant Production not elsewhere classified
970106 Expanding Knowledge in the Biological Sciences
060111 Signal Transduction
060703 Plant Developmental and Reproductive Biology
070108 Sustainable Agricultural Development
Abstract An ABA insensitive mutant, Beyma, was isolated in Lotus japonicus MG-20 from an EMS mutagenesis population using root growth inhibition to applied ABA as the screening criterion. (The name ‘Beyma’ was taken from the Australian Aboriginal language,Wagiman, beyma, meaning ‘drying up’.) The stable mutant that segregates as a dominant Mendelian mutation is insensitive to ABA induced inhibition of germination, vegetative growth, stomatal opening, as well as nodulation. Tissue ABA levelswere normal, suggesting a sensitivity rather than biosynthesismutation. It is slow-growing (50–70% of wild-type MG-20) and has a near-constitutive wilty phenotype associated with its inability to regulate stomatal opening. Whilst showing a wide range of ABA insensitive phenotypes, Beyma did not show alteration of nodule number control, as, in the absence of added ABA, the number and patterning (but not size) of nodules formed in the mutant were similar to that of MG-20. Split root experiments on MG-20 showed that application of ABA on one side of the root inhibited nodulation locally but not systemically. We propose that ABA is not involved directly in systemic autoregulation of nodulation (AON).
Formatted abstract
An ABA insensitive mutant, Beyma, was isolated in Lotus japonicus MG-20 from an EMS mutagenesis population
using root growth inhibition to applied ABA as the screening criterion. (The name ‘Beyma’ was taken from the
Australian Aboriginal language,Wagiman, beyma, meaning ‘drying up’.) The stable mutant that segregates as a dominant
Mendelian mutation is insensitive to ABA induced inhibition of germination, vegetative growth, stomatal opening, as well
as nodulation. Tissue ABA levelswere normal, suggesting a sensitivity rather than biosynthesismutation. It is slow-growing
(50–70% of wild-type MG-20) and has a near-constitutive wilty phenotype associated with its inability to regulate stomatal
opening. Whilst showing a wide range of ABA insensitive phenotypes, Beyma did not show alteration of nodule number
control, as, in the absence of added ABA, the number and patterning (but not size) of nodules formed in the mutant were
similar to that of MG-20. Split root experiments on MG-20 showed that application of ABA on one side of the root inhibited
nodulation locally but not systemically. We propose that ABA is not involved directly in systemic autoregulation of
nodulation (AON).
Keyword Hormone biology
water relations
genetics
development
symbiosis
legume
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
2010 Higher Education Research Data Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 08:04:13 EST by Mr Andrew Martlew on behalf of Centre for Integrative Legume Research