Respiratory burst function of ovine neutrophils

Tung, John-Paul, Fraser, John F., Wood, Peter and Fung, Yoke L. (2009) Respiratory burst function of ovine neutrophils. BMC Immunology, 10 25.1-25.11. doi:10.1186/1471-2172-10-25

Author Tung, John-Paul
Fraser, John F.
Wood, Peter
Fung, Yoke L.
Title Respiratory burst function of ovine neutrophils
Journal name BMC Immunology   Check publisher's open access policy
ISSN 1471-2172
Publication date 2009-05
Sub-type Article (original research)
DOI 10.1186/1471-2172-10-25
Open Access Status DOI
Volume 10
Start page 25.1
End page 25.11
Total pages 11
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2010
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
970111 Expanding Knowledge in the Medical and Health Sciences
110704 Cellular Immunology
Formatted abstract
Background: Respiratory burst function resulting in the release of reactive oxygen species such as superoxide anion (O2-) from neutrophils is one of the key mechanisms of the innate immune system, and maladaptive control of this mechanism is thought to play a pivotal role in the development of pathologies such as acute lung injury and sepsis. Ovine models of these pathologies are limited by the poor understanding of ovine neutrophil respiratory burst function.

Aspects of ovine neutrophil respiratory burst function to be characterised were: i) the maximum rate of O2- generated (Vmax); ii) the time taken to reach Vmax; iii) the total amount of O2- generated during the reaction; and iv) the duration of the reaction. As well as for unstimulated neutrophils, these aspects were also characterised after incubation with a priming agonist (platelet activating factor [PAF], tumour necrosis factor alpha [TNF-α] and lipopolysaccharides [LPS]) activating agonists (N-formylmethionyl-leucyl-phenylalanine [fMLP] and phorbol 12-myristate 13-acetate [PMA]) or a combination of a priming and an activating agonist. In the absence of priming or activating agonists, ovine neutrophils displayed a low level of respiratory burst function which was not enhanced by either PAF, TNF-α, LPS or fMLP, but was significantly enhanced by PMA. The PMA-induced respiratory burst function was further enhanced by pre-incubation with PAF, but not with TNF-α or LPS. By varying the length of pre-incubation with PAF it was demonstrated that this effect decreased as the duration of pre-incubation with PAF increased, and that PAF was enhancing PMA's effects rather than PMA enhancing PAF's effects.

Conclusion: This study successfully adapted a commonly used method of measuring human neutrophil respiratory burst function to characterise different aspects of ovine neutrophil respiratory burst function. This improved understanding of ovine neutrophils will facilitate the validitation of ovine biomedical models of human pathologies in which neutrophils have been implicated.
Keyword Platelet-activating factor
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number 25

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
ERA 2012 Admin Only
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 08:03:09 EST by Mr Andrew Martlew on behalf of Anaesthesiology and Critical Care - RBWH