The generation and properties of human macrophage populations from hemopoietic stem cells

Way, K. J., Dinh, H, Keene, M. R., White, K. E., Clanchy, F. I. L., Lusby, P, Roiniotis, J, Cook, A. D., Cassady, A. Ian, Curtis, D. J. and Hamilton, J. A. (2009) The generation and properties of human macrophage populations from hemopoietic stem cells. Journal of Leukocyte Biology, 85 5: 766-778. doi:10.1189/jlb.1108689

Author Way, K. J.
Dinh, H
Keene, M. R.
White, K. E.
Clanchy, F. I. L.
Lusby, P
Roiniotis, J
Cook, A. D.
Cassady, A. Ian
Curtis, D. J.
Hamilton, J. A.
Title The generation and properties of human macrophage populations from hemopoietic stem cells
Journal name Journal of Leukocyte Biology   Check publisher's open access policy
ISSN 0741-5400
Publication date 2009-05
Year available 2009
Sub-type Article (original research)
DOI 10.1189/jlb.1108689
Volume 85
Issue 5
Start page 766
End page 778
Total pages 13
Editor Joost J. Oppenheim
Place of publication Bethesda, MD, U. S. A.
Publisher Federation of American Societies for Experimental Biology
Collection year 2010
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
110707 Innate Immunity
Abstract Information about the development and function of human macrophage lineage populations, such as osteoclasts, is limited because of the lack of defined in vitro systems for their large-scale generation. Two M-CSF-containing cytokine cocktails were found under serum-free conditions to expand dramatically and to differentiate over time human CD34+ hemopoietic stem cells into nonadherent and adherent macrophage populations. These populations exhibited increasing degrees of maturity over a 3-week period characterized by morphology, surface marker expression (CD11b, CD86, CD64, CD14, and c-Fms), phagocytic function, and gene-expression profiling using quantitative PCR and microarray analysis (principal component analysis, k-means clustering, and gene ontology classification). As assessed by the last criterion, the adherent population obtained at 3 weeks from the one protocol tested had high similarity to the well-studied peripheral blood monocyte-derived macrophages. The one population tested could be induced to differentiate into osteoclasts in the presence of M-CSF and receptor activator of NF-{kappa}B ligand, as judged by morphology, gene expression, and bone-resorbing ability. In addition to the large numbers of macrophage lineage cells able to be produced, this replicating system may be suitable for the molecular analysis of macrophage lineage commitment and progression and for gene targeting and delivery.
Keyword M-CSF
Colony-stimulating Factor
gene expression profile
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Institute for Molecular Bioscience - Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 22 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 23 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 03 Sep 2009, 08:02:02 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience