Differing cell population structure reflects differing activity of Percoll-separated pronephros and peritoneal leucocytes from barramundi (Lates calcarifer)

Tumbol, RA, Baiano, JCF and Barnes, AC (2009) Differing cell population structure reflects differing activity of Percoll-separated pronephros and peritoneal leucocytes from barramundi (Lates calcarifer). AQUACULTURE, 292 3-4: 180-188. doi:10.1016/j.aquaculture.2009.04.030


Author Tumbol, RA
Baiano, JCF
Barnes, AC
Title Differing cell population structure reflects differing activity of Percoll-separated pronephros and peritoneal leucocytes from barramundi (Lates calcarifer)
Formatted title
Differing cell population structure reflects differing activity of Percoll-separated pronephros and peritoneal leucocytes from barramundi (Lates calcarifer)

Journal name AQUACULTURE   Check publisher's open access policy
ISSN 0044-8486
Publication date 2009-07-01
Sub-type Article (original research)
DOI 10.1016/j.aquaculture.2009.04.030
Volume 292
Issue 3-4
Start page 180
End page 188
Total pages 9
Editor B. A. Costa-Pierce
E. M. Donaldson
G. Hulata
Place of publication Netherlands
Publisher Elsevier BV
Collection year 2010
Language eng
Subject C1
830102 Aquaculture Fin Fish (excl. Tuna)
070401 Aquaculture
Formatted abstract
Aquaculture of barramundi or Asian sea bass (Lates calcarifer L) is expanding throughout the Asia-Pacific
region in both marine and freshwater systems. Incidence of bacterial and viral diseases is high in this species
throughout the region yet little is known about the immune system of this highly adaptable euryhaline fish.
Ultimately, most pathogens are eradicated by the phagocytic cells, however there is great diversity and
plasticity amongst these cell populations in mammals and in fish. To better understand disease processes in
barramundi, Percoll-purified leucocyte populations from haematopoietic tissues of the head kidney were
compared with populations isolated from the peritoneal cavity morphologically, cytochemically and in terms
of the ability to respond to stimulation, using flow cytometry, light microscopy and fluorimetric/
luminometric assays. The peritoneal cells comprised predominantly of macrophages and putative mature
monocytes whilst the head kidney cells comprised lymphocytes, including immunoglobulin-positive Blymphocytes,
some small monocytes and macrophages. The differing population structures were reflected in
the ability of the cells to respond to stimulation with either lipopolysaccharide or phorbol myristate acetate,
as the chemiluminescence response of peritoneal cells was 7 to 9-fold higher than head kidney cells. Both
populations were capable of being primed by LPS, but the kinetics differed, with optimal priming in
peritoneal cells occurring after 6 h exposure whilst head kidney cells required at least 24 h exposure to LPS
for optimal priming. Both head kidney and peritoneal populations produced nitric oxide in response to
stimulation with LPS and interferon gamma, but again, response was higher in peritoneal cells. The
implications of the differing population structures and activities amongst these cells should be considered
when developing models for further study of host–pathogen interactions in this increasingly important fish.
Keyword Barramundi
BASS DICENTRARCHUS-LABRAX
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
School of Biological Sciences Publications
Centre for Marine Studies Publications
 
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Created: Thu, 03 Sep 2009, 17:54:50 EST by Mr Andrew Martlew on behalf of Centre for Marine Studies