Micro-Raman spectroscopic observation of water expulsion induced destruction of hydrophobic clusters in crystalline lysozyme

Shou, Jing-Jing, Zeng, Guang, Zhang, Yun-Hong and Lu, G. Q (Max) (2009) Micro-Raman spectroscopic observation of water expulsion induced destruction of hydrophobic clusters in crystalline lysozyme. Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical, 113 29: 9633-9635. doi:10.1021/jp904257x


Author Shou, Jing-Jing
Zeng, Guang
Zhang, Yun-Hong
Lu, G. Q (Max)
Title Micro-Raman spectroscopic observation of water expulsion induced destruction of hydrophobic clusters in crystalline lysozyme
Journal name Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical   Check publisher's open access policy
ISSN 1520-6106
1520-5207
Publication date 2009-06
Sub-type Article (original research)
DOI 10.1021/jp904257x
Volume 113
Issue 29
Start page 9633
End page 9635
Total pages 3
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Formatted abstract
In this paper, three kinds of solid lysozyme samples with different water contents were investigated by confocal Raman spectroscopy. For the rod-like lysozyme crystal with highest water content, a sudden decrease of the intensity ratio of the doublet at 1338 and 1360 cm-1 was observed when the ambient relative humidity (RH) was lower than 86%, indicating the destruction of hydrophobic clusters of lysozyme induced by the expulsion of the hydration water from the crystal. In contrast to the rod-like crystal, tetragonal crystal and floor-like precipitate with a smaller amount of water showed no change of the structures of the hydrophobic clusters when the relative humidity was decreasing. The presence of bulk water in the rod-like crystal is believed a necessary factor for the function of the hydration water which promotes the hydrophobicity of hydrophobic clusters.
Keyword Residual structure
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Thu, 03 Sep 2009, 07:47:19 EST by Mr Andrew Martlew on behalf of Aust Institute for Bioengineering & Nanotechnology