Detection of BK, JC, WU, or KI polyomaviruses in faecal, urine, blood, cerebrospinal fluid and respiratory samples

Bialasiewicz, Seweryn, Whiley, David M., Lambert, Stephen B., Nissen, Michael D. and Sloots, Theo P. (2009) Detection of BK, JC, WU, or KI polyomaviruses in faecal, urine, blood, cerebrospinal fluid and respiratory samples. Journal of Clinical Virology, 45 3: 249-254. doi:10.1016/j.jcv.2009.05.002


Author Bialasiewicz, Seweryn
Whiley, David M.
Lambert, Stephen B.
Nissen, Michael D.
Sloots, Theo P.
Title Detection of BK, JC, WU, or KI polyomaviruses in faecal, urine, blood, cerebrospinal fluid and respiratory samples
Journal name Journal of Clinical Virology   Check publisher's open access policy
ISSN 1386-6532
Publication date 2009-07
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.jcv.2009.05.002
Volume 45
Issue 3
Start page 249
End page 254
Total pages 6
Editor Dr B Carman
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2010
Language eng
Subject 110309 Infectious Diseases
060506 Virology
060502 Infectious Agents
920203 Diagnostic Methods
920109 Infectious Diseases
C1
Abstract Background The recently described WU (WUV) and KI (KIV) polyomaviruses have been primarily detected in respiratory samples, however other body sites have not been extensively investigated to date. The related human polyomaviruses JCV and BKV in contrast, have been detected in a wide range of sample types, leading to increased knowledge about their biology and pathogenesis. Objectives The aim of the study was to investigate and compare the presence of JCV, BKV, WUV, and KIV in a variety of patient samples. Study design Nasopharyngeal aspirates (NPAs), bronchoalveolar lavages (BALs), cerebrospinal fluid (CSF), blood, faeces and urine from paediatric and adult immunocompetent and compromised patients were screened for the presence of the polyomaviruses by real-time PCR. The non-translated region (NTR) and VP1 of select WUV and KIV positive samples were sequenced and analysed. Results WUV and KIV were predominantly detected in NPA, BAL, and faeces from paediatric patients. JCV and BKV were primarily detected in blood, urine and faeces from adult patients. WUV and KIV NTR/VP1 sequence similarity ranged from 99.5% to 100% and 97.5–100%, respectively. Conclusions Overall, WUV and KIV were detected in paediatric respiratory tract samples, in contrast to JCV and BKV, in which respiratory detections were uncommon. Additionally, the lack of WUV and KIV detections in blood, CSF, urine and adult faeces reinforces the parallel in divergent genomic phylogeny and apparent tissue tropism between JCV and BKV, and WUV and KIV. NTR/VP1 sequence variation did not appear to be associated with site of WUV or KIV detection. Crown Copyright © 2009 Published by Elsevier B.V. All rights reserved.
Formatted abstract
Background
The recently described WU (WUV) and KI (KIV) polyomaviruses have been primarily detected in respiratory samples, however other body sites have not been extensively investigated to date. The related human polyomaviruses JCV and BKV in contrast, have been detected in a wide range of sample types, leading to increased knowledge about their biology and pathogenesis.
Objectives
The aim of the study was to investigate and compare the presence of JCV, BKV, WUV, and KIV in a variety of patient samples.
Study design
Nasopharyngeal aspirates (NPAs), bronchoalveolar lavages (BALs), cerebrospinal fluid (CSF), blood, faeces and urine from paediatric and adult immunocompetent and compromised patients were screened for the presence of the polyomaviruses by real-time PCR. The non-translated region (NTR) and VP1 of select WUV and KIV positive samples were sequenced and analysed.
Results
WUV and KIV were predominantly detected in NPA, BAL, and faeces from paediatric patients. JCV and BKV were primarily detected in blood, urine and faeces from adult patients. WUV and KIV NTR/VP1 sequence similarity ranged from 99.5% to 100% and 97.5–100%, respectively.
Conclusions
Overall, WUV and KIV were detected in paediatric respiratory tract samples, in contrast to JCV and BKV, in which respiratory detections were uncommon. Additionally, the lack of WUV and KIV detections in blood, CSF, urine and adult faeces reinforces the parallel in divergent genomic phylogeny and apparent tissue tropism between JCV and BKV, and WUV and KIV. NTR/VP1 sequence variation did not appear to be associated with site of WUV or KIV detection.
Crown Copyright © 2009 Published by Elsevier B.V. All rights reserved.

Keyword WU virus
Polymerase-chain-reaction
KI
JC
BK
Polyomavirus
Tissue tropism
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Thu, 03 Sep 2009, 07:45:39 EST by Mr Andrew Martlew on behalf of Clinical Medical Virology Centre