Detection of novel influenza A(H1N1) virus by real-time RT-PCR.

Whiley, David M., Bialasiewicz, Seweryn, Bletchly, Cheryl, Faux, Cassandra E., Harrower, Bruce, Gould, Allan R., Lambert, Stephen B., Nimmo, Graeme R., Nissen, Michael D. and Sloots, Theo P. (2009) Detection of novel influenza A(H1N1) virus by real-time RT-PCR.. Journal of Clinical Virology, 45 3: 203-204. doi:10.1016/j.jcv.2009.05.032

Author Whiley, David M.
Bialasiewicz, Seweryn
Bletchly, Cheryl
Faux, Cassandra E.
Harrower, Bruce
Gould, Allan R.
Lambert, Stephen B.
Nimmo, Graeme R.
Nissen, Michael D.
Sloots, Theo P.
Title Detection of novel influenza A(H1N1) virus by real-time RT-PCR.
Journal name Journal of Clinical Virology   Check publisher's open access policy
ISSN 1386-6532
Publication date 2009-07
Year available 2009
Sub-type Article (original research)
DOI 10.1016/j.jcv.2009.05.032
Volume 45
Issue 3
Start page 203
End page 204
Total pages 2
Editor Dr B. Carman
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Collection year 2010
Language eng
Subject 110309 Infectious Diseases
060506 Virology
060502 Infectious Agents
920115 Respiratory System and Diseases (incl. Asthma)
920109 Infectious Diseases
920203 Diagnostic Methods
Abstract Accurate and rapid diagnosis of novel influenza A(H1N1) infection is critical for minimising further spread through timely implementation of antiviral treatment and other public health based measures. In this study we developed two TaqMan-based reverse transcription PCR (RT-PCR) methods for the detection of novel influenza A(H1N1) virus targeting the haemagglutinin and neuraminidase genes. The assays were validated using 152 clinical respiratory samples, including 61 Influenza A positive samples, collected in Queenland, Australia during the years 2008 to 2009 and a further 12 seasonal H1N1 and H3N2 influenza A isolates collected from years 2000 to 2002. A wildtype swine H1N1 isolate was also tested. RNA from an influenza A(H1N1) virus isolate (Auckland, 2009) was used as a positive control. Overall, the results showed that the RT-PCR methods were suitable for sensitive and specific detection of novel influenza A(H1N1) RNA in human samples.
Keyword Influenza
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Special section: Novel 2009 influenza A H1N1 (swine variant)

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Created: Thu, 03 Sep 2009, 07:45:37 EST by Mr Andrew Martlew on behalf of Clinical Medical Virology Centre