Inhibitor of REL/NF-KB is regulated in Sydney rock oysters in response to specific double-stranded RNA and Vibrio alginolyticus, but the major immune anti-oxidants EcSOD and Prx6 are non-inducible

Green, Timothy and Barnes, Andrew (2009) Inhibitor of REL/NF-KB is regulated in Sydney rock oysters in response to specific double-stranded RNA and Vibrio alginolyticus, but the major immune anti-oxidants EcSOD and Prx6 are non-inducible. Fish and Shellfish Immunology, 27 2: 260-265. doi:10.1016/j.fsi.2009.05.005


Author Green, Timothy
Barnes, Andrew
Title Inhibitor of REL/NF-KB is regulated in Sydney rock oysters in response to specific double-stranded RNA and Vibrio alginolyticus, but the major immune anti-oxidants EcSOD and Prx6 are non-inducible
Formatted title
Inhibitor of REL/NF-KB is regulated in Sydney rock oysters in response to specific double-stranded RNA and Vibrio alginolyticus, but the major immune anti-oxidants EcSOD and Prx6 are non-inducible
Journal name Fish and Shellfish Immunology   Check publisher's open access policy
ISSN 1050-4648
1095-9947
Publication date 2009-08-01
Sub-type Article (original research)
DOI 10.1016/j.fsi.2009.05.005
Volume 27
Issue 2
Start page 260
End page 265
Total pages 6
Editor A. E. Ellis
C. J. Secombes,
Place of publication London, U.K.
Publisher Academic Press
Collection year 2010
Language eng
Subject C1
830104 Aquaculture Oysters
070401 Aquaculture
Formatted abstract
Invertebrates, such as oysters have mechanisms to recognize different microbial associated molecular patterns (MAMPs) and recognition of these antigens triggers signaling pathways for the transcription of immune-related proteins. Components of the Rel/NF-КB pathway have been identified in mollusks but the role of different MAMPs in the activation of this pathway is poorly understood. In the current study, the Sydney rock oyster (Saccostrea glomerata) was injected intramuscularly with a range of different MAMPs. Oysters were sampled over a 24 h period and the difference in expression of genes associated with immunity was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) using RNA extracted from hemocytes. The results showed that oysters can discriminate between different nucleic acids and double-stranded RNA sequences for activation of the Rel/NF-КB pathway in S. glomerata. Polyinosinic–polycytidylic acid (poly I:C) up-regulated expression of inhibitor of Rel/NF-КB (IКB) by 26-fold and interferon-inhibiting cytokine (IK) by 2.2-fold, whereas polycytidylic acid–polyguanylic acid (poly G:C) and double stranded Vibrio alginolyticus genomic DNA (dsDNA) did not. IКB was also up-regulated 3.8-fold higher for oysters stimulated with V. alginolyticus bacterial cells suggesting that lipopolysaccharide (LPS) also activates the Rel/NF-КB pathway. The expressions of extracellular superoxide dismutase (EcSOD) and peroxiredoxin 6 (Prx6) were not significantly affected by dsRNA, dsDNA or V. alginolyticus bacterial cells suggesting that these two genes, previously demonstrated to be important in the resistance of S. glomerata to disease are not directly inducible by these MAMPs. This is the first study investigating differences in expression kinetics of immune genes in response to challenge from different MAMPs in this economically important bivalve species.

Keyword Oyster
NF-KAPPA-B
Gene expression
Superoxide dismutase
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Thu, 03 Sep 2009, 17:44:19 EST by Mr Andrew Martlew on behalf of Centre for Marine Studies