20-kDa placental hGH-V has diminished diabetogenic and lactogenic activities compared with 22-kDa hGH-N while retaining antilipogenic activity

Vickers, M. H., Gilmour, S., Gertler, A., Breier, B. H., Tunny, K., Waters, M. J. and Gluckman, P. D. (2009) 20-kDa placental hGH-V has diminished diabetogenic and lactogenic activities compared with 22-kDa hGH-N while retaining antilipogenic activity. American Journal of Physiology - Endocrinology and Metabolism, 297 3: E629-E637. doi:10.1152/ajpendo.00221.2009


Author Vickers, M. H.
Gilmour, S.
Gertler, A.
Breier, B. H.
Tunny, K.
Waters, M. J.
Gluckman, P. D.
Title 20-kDa placental hGH-V has diminished diabetogenic and lactogenic activities compared with 22-kDa hGH-N while retaining antilipogenic activity
Journal name American Journal of Physiology - Endocrinology and Metabolism   Check publisher's open access policy
ISSN 0193-1849
Publication date 2009-09
Sub-type Article (original research)
DOI 10.1152/ajpendo.00221.2009
Volume 297
Issue 3
Start page E629
End page E637
Total pages 9
Editor Amira Klip
Place of publication Bethesda MD, U.S.A.
Publisher American Physiological Society
Collection year 2010
Language eng
Subject C1
920106 Endocrine Organs and Diseases (excl. Diabetes)
0604 Genetics
Abstract Placental human growth hormone-variant (hGH-V) and pituitary human growth hormone-N (hGH-N) are of identical size (22 kDa) but differ in 13 residues scattered throughout the protein. Several isoforms of GH are produced by the hGH-N and hGH-V genes including a 20-kDa hGH-V resulting from a 45-bp deletion caused by the use of an alternative acceptor site within exon 3. To date, the biological properties of the 20-kDa GH-V have not been characterized in vivo. Using young male Wistar rats fed either chow or a high-fat (HF) diet for 4 wk postweaning, we investigated the effect of 7 days treatment with either 22-kDa hGH-N, 20-kDa hGH-V (5 ug·g–1·day–1 sc), or vehicle on body composition and endocrine and metabolic profiles. Total body growth (absolute weight gain and linear growth trajectory) in the 20-kDa hGH-V-treated animals was intermediary between that of control and hGH-N-treated animals. Both 22-kDa hGH-N and 20-kDa hGH-V significantly reduced total body fat mass compared with control animals, and there were no differences between the GH isoforms in anti-lipogenic activity in animals fed the HF diet. Fasting plasma insulin and C peptide were significantly increased in animals on the HF diet and further increased by hGH-N but were unchanged in 20-kDa hGH-V-treated animals compared with saline-treated controls. Plasma volume as assessed by hematocrit was increased in hGH-N-treated animals but was unchanged in 20-kDa hGH-V-treated animals compared with controls. Furthermore, 20-kDa hGH-V had reduced lactogenic (prolactin receptor mediated) activity characteristic of hGH-N as tested in vitro compared with the 20-kDa hGH-N and 22-kDa hGH-N variants. In summary, placental 20-kDa hGH-V retains some of the growth-promoting and all antilipogenic activities of pituitary 22-kDa hGH-N but has diminished diabetogenic and lactogenic properties compared with the native 22-kDa hGH-N.
Keyword Placental growth hormone
Insulin sensitivity
Growth
Adiposity
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
ERA 2012 Admin Only
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Created: Thu, 03 Sep 2009, 07:43:11 EST by Mr Andrew Martlew on behalf of Institute for Molecular Bioscience