Photoreactivation is the main repair pathway for UV-induced DNA damage in coral planulae

Reef, R, Dunn, S, Levy, O, Dove, S, Shemesh, E, Brickner, I, Leggat, W and Hoegh-Guldberg, O (2009) Photoreactivation is the main repair pathway for UV-induced DNA damage in coral planulae. Journal of Experimental Biology, 212 17: 2760-2766. doi:10.1242/jeb.031286

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Author Reef, R
Dunn, S
Levy, O
Dove, S
Shemesh, E
Brickner, I
Leggat, W
Hoegh-Guldberg, O
Title Photoreactivation is the main repair pathway for UV-induced DNA damage in coral planulae
Journal name Journal of Experimental Biology   Check publisher's open access policy
ISSN 0022-0949
Publication date 2009-09
Sub-type Article (original research)
DOI 10.1242/jeb.031286
Open Access Status File (Publisher version)
Volume 212
Issue 17
Start page 2760
End page 2766
Total pages 7
Editor H. Hoppeler
Place of publication Cambridge, UK
Publisher The Company of Biologists
Collection year 2010
Language eng
Subject C1
060203 Ecological Physiology
960899 Flora, Fauna and Biodiversity of Environments not elsewhere classified
Abstract The larvae of most coral species spend some time in the plankton, floating just below the surface and hence exposed to high levels of ultraviolet radiation (UVR). The high levels of UVR are potentially stressful and damaging to DNA and other cellular components, such as proteins, reducing survivorship. Consequently, mechanisms to either shade (prevent) or repair damage potentially play an important role. In this study, the role of photoreactivation in the survival of coral planulae was examined. Photoreactivation is a light-stimulated response to UV-damaged DNA in which photolyase proteins repair damaged DNA. Photoreactivation rates, as well as the localization of photolyase, were explored in planulae under conditions where photoreactivation was or was not inhibited. The results indicate that photoreactivation is the main DNA repair pathway in coral planulae, repairing UV-induced DNA damage swiftly (K=1.75 h–1 and a half-life of repair of 23 min), with no evidence of any light-independent DNA repair mechanisms, such as nucleotide excision repair (NER), at work. Photolyase mRNA was localized to both the ectoderm and endoderm of the larvae. The amount of cell death in the coral planulae increased significantly when photoreactivation was inhibited, by blocking photoreactivating light. We found that photoreactivation, along with additional UV shielding in the form of five mycosporine-like amino acids, are sufficient for survival in surface tropical waters and that planulae do not accumulate DNA damage despite being exposed to high UVR.
Keyword photolyase
Acropora millepora
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2010 Higher Education Research Data Collection
Centre for Marine Studies Publications
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Citation counts: TR Web of Science Citation Count  Cited 16 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 03 Sep 2009, 07:42:07 EST by Mr Andrew Martlew on behalf of Centre for Marine Studies