The effects of saltwater acclimation on Na-K-2Cl and Na-K-ATPase distribution, activity and gene expression in salt glands of the estuarine crocodile

Cramp, R., Husdon, N. and Franklin, C. (2008). The effects of saltwater acclimation on Na-K-2Cl and Na-K-ATPase distribution, activity and gene expression in salt glands of the estuarine crocodile. In: Comparative Biochemistry and Physiology A Molecular & Integrative Physiology. Annual Meeting of the Society for Experimental Biology, Marseille, France, (S105-S105). 6-10 July, 2008. doi:10.1016/j.cbpa.2008.04.219


Author Cramp, R.
Husdon, N.
Franklin, C.
Title of paper The effects of saltwater acclimation on Na-K-2Cl and Na-K-ATPase distribution, activity and gene expression in salt glands of the estuarine crocodile
Conference name Annual Meeting of the Society for Experimental Biology
Conference location Marseille, France
Conference dates 6-10 July, 2008
Proceedings title Comparative Biochemistry and Physiology A Molecular & Integrative Physiology   Check publisher's open access policy
Journal name Comparative Biochemistry and Physiology A-Molecular   Check publisher's open access policy
Place of Publication New York, U.S.
Publisher Elsevier
Publication Year 2008
DOI 10.1016/j.cbpa.2008.04.219
ISSN 1095-6433
Volume 150
Issue 3
Start page S105
End page S105
Total pages 1
Language eng
Formatted Abstract/Summary
The estuarine crocodile, Crocodylus porosus utilises lingual salt glands to excrete excess NaCl accumulated whilst living in saline environments. In avian salt gland models, Na–K-ATPase (NKA) is essential for the maintenance of the electrochemical gradients which facilitate the secretory activity of other ion pumps, while Na–K–2Cl (NKCC) functions to pump Na+ and K+ into the paracellular space, in exchange for Cl− which is transported into the cell. We have investigated the effects of chronic saltwater acclimation on the distribution, activity and gene expression patterns of both NKA and NKCC in the crocodilian salt gland. Using immunohistochemistry, both NKA and NKCC were localized to the basolateral membranes of salt gland secretory cells in both freshwater (fw) and saltwater (sw) acclimated salt gland tissues. The intensity of staining was slightly, but significantly, higher in tissues from SW-acclimated animals. Western blotting indicated that there was an increase in NKA and NKCC abundances in SW-acclimated tissues. The relative metabolic costs associated with these two pumps were investigated in vitro. While there were no differences between fw- and sw-acclimated tissues in the relative costs associated with NKA activity, NKCC activity was almost 50% higher in SW-acclimated tissues. We are currently exploring patterns of NKA and NKCC gene expression in sw- and fw-acclimated animals. This study has demonstrated the presence of NKA and NKCC in the salt glands of C. porosus and has shown that the activity and abundance of these transporters can be influenced by prolonged saltwater acclimation. Copyright © 2008 Published by Elsevier Inc.
Subjects EX
970106 Expanding Knowledge in the Biological Sciences
0606 Physiology
Keyword Crocodylus porosus
Salt glands
Saltwater acclimation
Q-Index Code EX
Q-Index Status Provisional Code

 
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Created: Tue, 07 Jul 2009, 10:59:27 EST by Hayley Ware on behalf of School of Biological Sciences