Decreased T cell reactivity to Epstein–Barr virus infected lymphoblastoid cell lines in multiple sclerosis

Pender, Michael P., Csurhes, Peter A., Lenarczyk, Aleksandra, Pfluger, Casey M. M. and Burrows, S. R. (2009) Decreased T cell reactivity to Epstein–Barr virus infected lymphoblastoid cell lines in multiple sclerosis. Journal of Neurology, Neurosurgery and Psychiatry, 80 5: 498-505. doi:10.1136/jnnp.2008.161018


Author Pender, Michael P.
Csurhes, Peter A.
Lenarczyk, Aleksandra
Pfluger, Casey M. M.
Burrows, S. R.
Title Decreased T cell reactivity to Epstein–Barr virus infected lymphoblastoid cell lines in multiple sclerosis
Formatted title
Decreased T cell reactivity to Epstein-Barr virus infected lymphoblastoid cell lines in multiple sclerosis
Journal name Journal of Neurology, Neurosurgery and Psychiatry   Check publisher's open access policy
ISSN 0022-3050
Publication date 2009
Sub-type Article (original research)
DOI 10.1136/jnnp.2008.161018
Volume 80
Issue 5
Start page 498
End page 505
Total pages 8
Place of publication London, England
Publisher BMJ Publishing Group
Collection year 2009
Language eng
Subject C1
110319 Psychiatry (incl. Psychotherapy)
1103 Clinical Sciences
1109 Neurosciences
1107 Immunology
110703 Autoimmunity
110904 Neurology and Neuromuscular Diseases
110903 Central Nervous System
Formatted abstract
Objective: To investigate T cell and antibody immunity to Epstein–Barr virus (EBV) in multiple sclerosis (MS).

Methods: Immunoglobulin G (IgG) immunity to EBV nuclear antigen 1 (EBNA1) and viral capsid antigen was measured by enzyme linked immunosorbent assays, and T cell immunity was assessed using enzyme linked immunospot assays to measure the frequency of peripheral blood mononuclear cells (PBMC) producing interferon γ in response to autologous EBV infected B cell lymphoblastoid cell lines (LCL) in 34 EBV seropositive healthy subjects and 34 EBV seropositive patients with MS who had not received immunomodulatory therapy in the previous 3 months.

Results: Patients with MS had increased levels of anti-EBNA1 IgG but a decreased frequency of LCL specific T cells compared with healthy subjects. Using purified populations of CD4+ T cells and CD8+ T cells, we showed that the LCL specific response resides predominantly in the CD8+ population, with a frequency 5–7-fold higher than in the CD4+ population. The decreased CD8+ T cell response to LCL in MS was not caused by decreased HLA class I expression by LCL, and LCL from MS patients could be killed normally by HLA matched EBV specific cytotoxic CD8+ T cell clones from healthy subjects. Furthermore, the decreased CD8+ T cell immunity to EBV was not due to a primary defect in the function of CD8+ T cells because EBV specific cytotoxic CD8+ T cell lines could be generated normally from the PBMC of patients with MS.

Conclusion: This quantitative deficiency in CD8+ T cell immunity to EBV might be responsible for the accumulation of EBV infected B cells in the brains of patients with MS.                               

Keyword Anti-body-titers
Immune-response
Nuclear antigen
EBV
Epitope
Lymphocytes
Immunodominance
Immunology
Disability
Frequency
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
UQ Centre for Clinical Research Publications
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 47 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 15 Jun 2009, 13:51:30 EST by Cameron Harris on behalf of Faculty Of Health Sciences