Extreme arsenic resistance by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1

Baker-Austin, Craig, Dopson, Mark, Wexler, Margaret, Sawers, R. Gary, Stemmler, Ann, Rosen, Barry P. and Bond, Philip L. (2007) Extreme arsenic resistance by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1. Extremophiles, 11 3: 425-434. doi:10.1007/s00792-006-0052-z

Author Baker-Austin, Craig
Dopson, Mark
Wexler, Margaret
Sawers, R. Gary
Stemmler, Ann
Rosen, Barry P.
Bond, Philip L.
Title Extreme arsenic resistance by the acidophilic archaeon 'Ferroplasma acidarmanus' Fer1
Journal name Extremophiles   Check publisher's open access policy
ISSN 1431-0651
Publication date 2007-05
Sub-type Article (original research)
DOI 10.1007/s00792-006-0052-z
Volume 11
Issue 3
Start page 425
End page 434
Total pages 10
Place of publication Tokyo
Publisher Springer Japan
Language eng
Subject 060599 Microbiology not elsewhere classified
Formatted abstract
‘Ferroplasma acidarmanus’ Fer1 is an arsenic-hypertolerant acidophilic archaeon isolated from the Iron Mountain mine, California; a site characterized by heavy metals contamination. The presence of up to 10 g arsenate per litre [As(V); 133 mM] did not significantly reduce growth yields, whereas between 5 and 10 g arsenite per litre [As(III); 67–133 mM] significantly reduced the yield. Previous bioinformatic analysis indicates that ‘F. acidarmanus’ Fer1 has only two predicted genes involved in arsenic resistance and lacks a recognizable gene for an arsenate reductase. Biochemical analysis suggests that ‘F. acidarmanus’ Fer1 does not reduce arsenate indicating that ‘F. acidarmanus’ Fer1 has an alternative resistance mechanism to arsenate other than reduction to arsenite and efflux. Primer extension analysis of the putative ars transcriptional regulator (arsR) and efflux pump (arsB) demonstrated that these genes are co-transcribed, and expressed in response to arsenite, but not arsenate. Two-dimensional polyacrylamide gel electrophoresis analysis of ‘F. acidarmanus’ Fer1 cells exposed to arsenite revealed enhanced expression of proteins associated with protein refolding, including the thermosome Group II HSP60 family chaperonin and HSP70 DnaK type heat shock proteins. This report represents the first molecular and proteomic study of arsenic resistance in an acidophilic archaeon.
Keyword Extremophiles
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 38 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 44 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 05 Jun 2009, 15:01:02 EST by Gina Velli on behalf of School of Chemistry & Molecular Biosciences