Effects of 17beta-estradiol and progesterone on transcription of human papillomavirus 16 E6/E7 oncogenes in CaSki and SiHa cell lines

Ruutu, M., Wahlroos, N., Syrjänen, K., Johansson, B. and Syrjänen, S. (2006) Effects of 17beta-estradiol and progesterone on transcription of human papillomavirus 16 E6/E7 oncogenes in CaSki and SiHa cell lines. International Journal of Gynecological Cancer, 16 3: 1261-1268. doi:10.1111/j.1525-1438.2006.00563.x


Author Ruutu, M.
Wahlroos, N.
Syrjänen, K.
Johansson, B.
Syrjänen, S.
Title Effects of 17beta-estradiol and progesterone on transcription of human papillomavirus 16 E6/E7 oncogenes in CaSki and SiHa cell lines
Formatted title
Effects of 17β-estradiol and progesterone on transcription of human papillomavirus 16 E6/E7 oncogenes in CaSki and SiHa cell lines
Journal name International Journal of Gynecological Cancer   Check publisher's open access policy
ISSN 1048-891X
1525-1438
Publication date 2006-05
Sub-type Article (original research)
DOI 10.1111/j.1525-1438.2006.00563.x
Volume 16
Issue 3
Start page 1261
End page 1268
Total pages 8
Place of publication New York, NY, United States
Publisher Lippincott Williams & Wilkins
Language eng
Subject 111201 Cancer Cell Biology
Abstract Several in vitro studies have addressed the interactions between estrogen/progesterone and human papillomavirus (HPV), but the results are controversial. We evaluated the effects of estrogen and progesterone and their antagonists on messenger RNA expression of HPV16 E6/E7 in HPV16-positive cell lines CaSki and SiHa with real-time reverse-transciptase polymerase chain reaction method. Colorimetric assay with tetrazolium salt (WST-1) and flow cytometry were used for testing proliferation and apoptosis. No statistically significant changes were found after hormone treatment in the expression of HPV16 E6/E7 or hormone receptors in CaSki and SiHa cell lines. Progesterone increased cell proliferation in both the cells, while estrogen increased proliferation of SiHa cells only. Estrogen seemed to protect the CaSki cells from apoptosis, and tamoxifen did not abrogate this effect. Progesterone slightly increased apoptosis of CaSki cells, and this effect was neutralized with RU486. In this study, estrogen and progesterone did not change either the transcription levels of HPV16 E6/E7 or estrogen receptor or progesterone receptor levels. Hormone receptor antagonists had no effect on transcription. Both hormones might have a permissive effect for the growth of cervical cancer, by promoting cell proliferation and making the cells vulnerable to mutations. In addition, estrogen acts as an antiapoptotic agent allowing growth advance of the cells infected with oncogenic HPV.
Keyword estrogen
E6/E7
gene expression
HPV
progesterone
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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