Treatment of spinal cord injury with co-grafts of genetically modified Schwann cells and fetal spinal cord cell suspension in the rat

Feng, Shi-Qing, Kong, Xiao-Hong, Guo, Shi-Fu, Wang, Pei, Li, Li, Zhong, Jin-Hua and Zhou, Xin-Fu (2005) Treatment of spinal cord injury with co-grafts of genetically modified Schwann cells and fetal spinal cord cell suspension in the rat. Neurotoxicity Research, 7 1-2: 169-177. doi:10.1007/BF03033785


Author Feng, Shi-Qing
Kong, Xiao-Hong
Guo, Shi-Fu
Wang, Pei
Li, Li
Zhong, Jin-Hua
Zhou, Xin-Fu
Title Treatment of spinal cord injury with co-grafts of genetically modified Schwann cells and fetal spinal cord cell suspension in the rat
Journal name Neurotoxicity Research   Check publisher's open access policy
ISSN 1476-3524
1029-8428
Publication date 2005-01
Sub-type Article (original research)
DOI 10.1007/BF03033785
Volume 7
Issue 1-2
Start page 169
End page 177
Total pages 9
Place of publication New York, NY, United States
Publisher Springer
Language eng
Subject 110904 Neurology and Neuromuscular Diseases
Formatted abstract
Fetal spinal cord cells, Schwann cells and neurotrophins all have the capacity to promote repair of injured spinal cord in animal models. To explore the possibility of using these approaches to treat clinical patients, we have examined whether a combination of these protocols produces functional and anatomical improvement. The spinal cords of adult rats (n=16) were injured with a modified New York University (NYU) device (10 gram.5cm). One week after injury, the injured cords were injected with Dulbecco-modified Eagles Medium (DMEM, control group), or fetal spinal cord cell suspension (FSCS) plus nerve growth factor (NGF) gene-modified Schwann cells (SC) and brain-derived neurotrophic factor (BDNF) genemodified SC (treatment group). The rats were subjected to BBB (Basso, Beattie, Bresnahan, Exp. Neurol. 139:244, 1996) behavioral tests. Anterograde tracing of corticospinal tract was performed before sacrifice 3 months after the treatment. The results showed that the combination treatment elicited a robust growth of corticospinal axons within and beyond the injury site. A dramatic functional recovery in the treatment group was observed compared with the control group. We conclude that the combination of FSCS with genetically modified Schwann cells over-expressing NGFand BDNF was an effective protocol for the treatment of severe spinal cord injury.
Keyword Schwann cell
Fetal spinal cord cells
Regeneration
Nerve growth factor
Brain-derived neurotrophic factor
Corticospinal tract
Behavior
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
 
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Created: Fri, 17 Apr 2009, 13:06:27 EST by Ms Karen Naughton on behalf of Queensland Brain Institute