Seeligeriolysin O, a Cholesterol-Dependent Cytolysin of Listeria seeligeri, Induces Gamma Interferon from Spleen Cells of Mice

Ito, Yutaka, Kawamura, Ikuo, Kohda, Chikara, Baba, Hisashi, Nomura, Takamasa, Kimoto, Terumi, Watanabe, Isao and Mitsuyama, Masao (2003) Seeligeriolysin O, a Cholesterol-Dependent Cytolysin of Listeria seeligeri, Induces Gamma Interferon from Spleen Cells of Mice. Infection and Immunity, 71 1: 234-241. doi:10.1128/IAI.71.1.234-241.2003

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Author Ito, Yutaka
Kawamura, Ikuo
Kohda, Chikara
Baba, Hisashi
Nomura, Takamasa
Kimoto, Terumi
Watanabe, Isao
Mitsuyama, Masao
Title Seeligeriolysin O, a Cholesterol-Dependent Cytolysin of Listeria seeligeri, Induces Gamma Interferon from Spleen Cells of Mice
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 1098-5522
1070-6313
Publication date 2003-01
Sub-type Article (original research)
DOI 10.1128/IAI.71.1.234-241.2003
Open Access Status File (Publisher version)
Volume 71
Issue 1
Start page 234
End page 241
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Seeligeriolysin O (LSO), one of the cholesterol-dependent cytolysins produced by Listeria seeligeri, shows 80% homology to listeriolysin O (LLO) produced by Listeria monocytogenes at the amino acid sequence level. In addition to cytolytic activity, LLO has been shown to exhibit cytokine-inducing activity. In order to determine whether LSO is also capable of exhibiting these two different activities, we constructed a recombinant full-length LSO (rLSO530) and a noncytolytic truncated derivative with a C-terminal deletion (rLSO483) and compared these molecules with recombinant LLO. The cytolytic rLSO530 molecule could induce gamma interferon (IFN-{gamma}) production in spleen cells when the cytolytic activity was blocked by treatment with cholesterol. The noncytolytic truncated rLSO483 molecule also induced IFN-{gamma} production. Anti-LLO polyclonal antibody inhibited not only LLO-induced IFN-{gamma} production but also LSO-induced IFN-{gamma} production. Both NK cells and CD11b+ cells were required for LSO-induced IFN-{gamma} production. Among the various cytokines expressed in CD11b+ cells, interleukin-12 (IL-12) and IL-18 appeared to be essential. We concluded that LSO exhibits the same biological activity as LLO.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Thu, 16 Apr 2009, 14:37:28 EST by Ms Sarada Rao on behalf of Anaesthesiology and Critical Care - RBWH