Amino acid availability determines the ratio of microcystin variants in the cyanobacterium planktothrix agardhii

Tonk, Linda, van de Waal, Dedmer B., Slot, Pieter, Huisman, Jef, Matthijs, Hans C. P. and Visser, Petra M. (2008) Amino acid availability determines the ratio of microcystin variants in the cyanobacterium planktothrix agardhii. FEMS Microbiology Ecology, 65 3: 383-390.


Author Tonk, Linda
van de Waal, Dedmer B.
Slot, Pieter
Huisman, Jef
Matthijs, Hans C. P.
Visser, Petra M.
Title Amino acid availability determines the ratio of microcystin variants in the cyanobacterium planktothrix agardhii
Formatted title  Amino acid availability determines the ratio of microcystin variants in the cyanobacterium Planktothrix agardhii
Journal name FEMS Microbiology Ecology   Check publisher's open access policy
ISSN 0168-6496
1574-6941
Publication date 2008-09
Sub-type Article (original research)
DOI 10.1111/j.1574-6941.2008.00541.x
Volume 65
Issue 3
Start page 383
End page 390
Total pages 8
Editor J. I. Prosser
Riks Laanbroek
Place of publication Oxford, U.K.
Publisher Wiley-Blackwell Publishing
Collection year 2009
Language eng
Subject C1
960808 Marine Flora, Fauna and Biodiversity
060205 Marine and Estuarine Ecology (incl. Marine Ichthyology)
06 Biological Sciences
0607 Plant Biology
0605 Microbiology
Formatted abstract Cyanobacteria are capable of producing multiple microcystin variants simultaneously. The mechanisms that determine the composition of microcystin variants in cyanobacteria are still debated. [Asp3]microcystin-RR contains arginine at the position where the more toxic [Asp3]microcystin-LR incorporates leucine. We cultured the filamentous cyanobacterium Planktothrix agardhii strain 126/3 with and without external addition of leucine and arginine. Addition of leucine to the growth medium resulted in a strong increase of the [Asp3]microcystin LR/RR ratio, while addition of arginine resulted in a decrease. This demonstrates that amino acid availability plays a role in the synthesis of different microcystin variants. Environmental changes affecting cell metabolism may cause differences in the intracellular availability of leucine and arginine, which can thus affect the production of microcystin variants. Because leucine contains one nitrogen atom while arginine contains four nitrogen atoms, we hypothesized that low nitrogen availability might shift the amino acid composition in favor of leucine, which might explain seasonal increases in the [Asp3]microcystin LR/RR ratio in natural populations. However, when a continuous culture of P. agardhii was shifted from nitrogen-saturated to a nitrogen-limited mineral medium, leucine and arginine concentrations decreased, but the leucine/arginine ratio did not change. Accordingly, while the total microcystin concentration of the cells decreased, we did not observe changes in the [Asp3]microcystin LR/RR ratio in response to nitrogen limitation.
Keyword Amino acid
Microcystin
Cyanobacterium
Planktothrix
Agardhii
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Original text in Dutch.

 
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