Community proteogenomics highlights microbial strain-variant protein expression within activated sludge performing enhanced biological phosphorus removal

Wilmes, Paul, Andersson, Anders F., Lefsrud, Mark G., Wexler, Margaret, Shah, Manesh, Zhang, Bing, Hettich, Robert L, Bond, Philip L., VerBerkmoes, Nathan C. and Banfield, Jillian F. (2008) Community proteogenomics highlights microbial strain-variant protein expression within activated sludge performing enhanced biological phosphorus removal. ISME Journal, 2 8: 853-864. doi:10.1038/ismej.2008.38


Author Wilmes, Paul
Andersson, Anders F.
Lefsrud, Mark G.
Wexler, Margaret
Shah, Manesh
Zhang, Bing
Hettich, Robert L
Bond, Philip L.
VerBerkmoes, Nathan C.
Banfield, Jillian F.
Title Community proteogenomics highlights microbial strain-variant protein expression within activated sludge performing enhanced biological phosphorus removal
Journal name ISME Journal   Check publisher's open access policy
ISSN 1751-7362
Publication date 2008
Year available 2008
Sub-type Article (original research)
DOI 10.1038/ismej.2008.38
Volume 2
Issue 8
Start page 853
End page 864
Total pages 12
Place of publication New York
Publisher Nature Publishing Group
Collection year 2009
Language eng
Subject C1
9699 Other Environment
090409 Wastewater Treatment Processes
Formatted abstract
Enhanced biological phosphorus removal (EBPR) selects for polyphosphate accumulating microorganisms to achieve phosphate removal from wastewater. We used high-resolution community proteomics to identify key metabolic pathways in ‘Candidatus Accumulibacter phosphatis’ (A. phosphatis)-mediated EBPR and to evaluate the contributions of co-existing strains within the dominant population. Overall, 702 proteins from the A. phosphatis population were identified. Results highlight the importance of denitrification, fatty acid cycling and the glyoxylate bypass in EBPR. Strong similarity in protein profiles under anaerobic and aerobic conditions was uncovered (only 3% of A. phosphatis-associated proteins exhibited statistically significant abundance differences). By comprehensive genome-wide alignment of 13 930 orthologous proteins, we uncovered substantial differences in protein abundance for enzyme variants involved in both core-metabolism and EBPR-specific pathways among the A. phosphatis population. These findings suggest an essential role for genetic diversity in maintaining the stable performance of EBPR systems and, hence, demonstrate the power of integrated cultivation-independent genomics and proteomics for the analysis of complex biotechnological systems.
Keyword ‘Candidatus Accumulibacter phosphatis’
community proteogenomics
enhanced biological phosphorus removal
metaproteomics
proteomics
strain variation
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Thu, 16 Apr 2009, 11:45:02 EST by Suzanne Read on behalf of Advanced Water Management Centre