Evaluation of a Commercially available Serologic assay for Antibodies against Tuberculosis-associated Glycolipid Antigen

Iinuma, Yoshitsugu, Senda, Kazuyoshi, Takakura, Shunji, Ichiyama, Satoshi, Tano, Masao, Abe, Tomoji, Yamamoto, Tomoko, Nakashima, Kazumitsu, Baba, Hisashi, Hasegawa, Yoshinori and Shimokata, Kaoru (2002) Evaluation of a Commercially available Serologic assay for Antibodies against Tuberculosis-associated Glycolipid Antigen. Clinical Chemistry And Laboratory Medicine, 40 8: 832-836. doi:10.1515/CCLM.2002.144

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Author Iinuma, Yoshitsugu
Senda, Kazuyoshi
Takakura, Shunji
Ichiyama, Satoshi
Tano, Masao
Abe, Tomoji
Yamamoto, Tomoko
Nakashima, Kazumitsu
Baba, Hisashi
Hasegawa, Yoshinori
Shimokata, Kaoru
Title Evaluation of a Commercially available Serologic assay for Antibodies against Tuberculosis-associated Glycolipid Antigen
Journal name Clinical Chemistry And Laboratory Medicine   Check publisher's open access policy
ISSN 1434-6621
1437-4331
Publication date 2002-08
Year available 2005
Sub-type Article (original research)
DOI 10.1515/CCLM.2002.144
Open Access Status File (Publisher version)
Volume 40
Issue 8
Start page 832
End page 836
Total pages 5
Place of publication The Hague
Publisher Walter de Gruyter & Co.
Language eng
Subject 110303 Clinical Microbiology
Abstract A commercially available enzyme immunoassay developed to detect antibodies to a tuberculosis-associated glycolipid antigen was evaluated for serologic diagnosis of tuberculosis. This was a multicenter study comparing the assay with other methods in 78 patients with active pulmonary tuberculosis and in 54 controls with non-tuberculous lung diseases. Sensitivities were highest for sputum culture (91.0%), followed by immunoassay (79.5%), nucleic acid amplification (77.3%), and finally acid-fast staining of sputum smear (60.3%). Immunoassay and amplification, both rapid methods, had similarly high sensitivity in smear-positive subjects (89.4 and 88.9%, respectively); in smear-negative subjects these two techniques showed low sensitivity (64.5 and 60.0%, respectively). Concordance between the two methods was relatively low (72.0%). With regard to specificity, seven out of ten patients with old tuberculosis had positive result by immunoassay (30% specificity). In the control group, 10 out of 54 patients had positive immunoassay result (72.2% specificity), with notably limited specificity in the elderly. The tuberculous glycolipid assay is a rapid method sufficiently sensitive for detection of tuberculosis infection, even in smear-negative patients.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Published Online: 01/06/2005

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Wed, 08 Apr 2009, 11:50:26 EST by Juliette Grosvenor on behalf of Anaesthesiology and Critical Care - RBWH