Evaluation of Automated Ribotyping System for Characterization and Identification of Verocytotoxin-Producing Escherichia coli Isolated in Japan

Ito, Yuji, Iinuma, Yoshitsugu, Baba, Hisashi, Sugino, Yasuteru, Hawagawa, Yoshinori, Shimokata, Kaoru, Ichiyama, Satoshi, Hasegawa, Tadao and Ohta, Michio (2003) Evaluation of Automated Ribotyping System for Characterization and Identification of Verocytotoxin-Producing Escherichia coli Isolated in Japan. Japanese Journal of Infectious Diseases, 56 5-6: 200-204.

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Author Ito, Yuji
Iinuma, Yoshitsugu
Baba, Hisashi
Sugino, Yasuteru
Hawagawa, Yoshinori
Shimokata, Kaoru
Ichiyama, Satoshi
Hasegawa, Tadao
Ohta, Michio
Title Evaluation of Automated Ribotyping System for Characterization and Identification of Verocytotoxin-Producing Escherichia coli Isolated in Japan
Formatted title
Evaluation of Automated Ribotyping System for Characterization
and Identification of Verocytotoxin-Producing Escherichia coli
Isolated in Japan
Journal name Japanese Journal of Infectious Diseases   Check publisher's open access policy
ISSN 1344-6304
Publication date 2003
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 56
Issue 5-6
Start page 200
End page 204
Total pages 5
Editor Ichiro Kurane
Place of publication Tokyo, Japan
Publisher National Institute of Infectious Diseases
Language eng
Subject 110303 Clinical Microbiology
110309 Infectious Diseases
Formatted abstract
The usefulness of an automated ribotyping system (RiboPrinter) was evaluated for characterizing and identifying clinical isolates of 37 verocytotoxin-producing Escherichia coli (VTEC) strains and 16 non-VTEC strains. All strains were successfully ribotyped with satisfactory reproducibility and stability and characterized into 10 different ribogroups. All VTEC O157 strains were characterized into a specific ribogroup and correctly typed into the specific DuPont ID for VTEC O157:H7, while all of the non-VTEC O157 strains were clearly distinguished from VTEC O157. VTEC O26 and O111 strains, the most prevalent VTEC serotypes after O157, were also well characterized into specific ribogroups and identified. These results suggest that the RiboPrinter may have an advantage over other typing systems in that it can rapidly and easily discriminate VTEC from non-VTEC strains of the most prevalent VTEC serotypes in Japan, even though it provides a lesser degree of discrimination than pulsed-field gel electrophoresis (PFGE). With a hierarchical or sequential typing combining the RiboPrinter and PFGE, rapid and accurate typing can be achieved during an outbreak of VTEC, which may be useful in clinical and public health settings.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Wed, 08 Apr 2009, 11:16:53 EST by Juliette Grosvenor on behalf of Anaesthesiology and Critical Care - RBWH