Microdomain Ca2+ dynamics in mammalian muscle following prolonged high pressure treatments

Schnee, S., vWegner, F., Schurmann, S., Ludwig, H., Fink, R. H. A. and Friedrich, O. (2008) Microdomain Ca2+ dynamics in mammalian muscle following prolonged high pressure treatments. Journal of Physics: Conference Series, 121 1: 1-10. doi:10.1088/1742-6596/121/1/112003


Author Schnee, S.
vWegner, F.
Schurmann, S.
Ludwig, H.
Fink, R. H. A.
Friedrich, O.
Title Microdomain Ca2+ dynamics in mammalian muscle following prolonged high pressure treatments
Formatted title
Microdomain Ca2+ dynamics in mammalian muscle following prolonged high pressure treatments
Journal name Journal of Physics: Conference Series   Check publisher's open access policy
ISSN 1742-6596
1742-6588
Publication date 2008
Year available 2008
Sub-type Article (original research)
DOI 10.1088/1742-6596/121/1/112003
Open Access Status DOI
Volume 121
Issue 1
Start page 1
End page 10
Total pages 10
Place of publication Bristol, United Kingdom
Publisher Institu of Physics Publishing
Collection year 2009
Language eng
Subject C1
060114 Systems Biology
920199 Clinical Health (Organs, Diseases and Abnormal Conditions) not elsewhere classified
Formatted abstract
High pressure (HP) applications are an important thermodynamic tool to infuence cellular processes. Especially processes that undergo large volume changes, e.g. opening or closing of ion channels, are in particular susceptible to HP treatments. Such volume changes are extremely di±cult to assess for intracellular ion channels, like ryanodine receptors (RyR) residing in the membrane of organelles. In skeletal muscle, RyR act as Ca 2+ release channels. We previously showed that plasmalemmal Na + and Ca 2+ ion channels were irreversibly altered after prolonged 20 MPa treatments. Here, changes in microdomain Ca 2+ levels due to elementary Ca 2+ release events (ECRE) were monitored using confocal fluorescence microscopy. We studied ECRE in mammalia skeletal muscle following 3 h HP treatments up to 30  MPa to clarify whether RyR induced intracellular microdomain Ca 2+ dynamics was more susceptible to HP treatment compared to surface membrane ion currents. ECRE frequencies exponentially declined with pressure. ECRE amplitudes and rise times (RT) were quite robust towards HP treatments. In contrast, spatial and temporal ECRE extension showed a tendency towardslarger values up to  20 MPa but declined for higher pressures. Activation volumes for pressure-induced persistent ECRE alterations were zero for RT but showed a bimodal behavior for event duration. It seems that although ECRE frequencies are markedly reduced, ECRE morphology is less affected by HP. In particular, RyR opening time is practically unaltered and the observed morphological ECRE changes might reflect alterations in local Ca 2+ buffers and Ca 2+ concentration profiles rather than involvement of RyR in mammalian skeletal muscle.  
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
ERA 2012 Admin Only
School of Biomedical Sciences Publications
 
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Created: Tue, 07 Apr 2009, 15:46:50 EST by Shirley Rey on behalf of School of Biomedical Sciences