Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahai grass Paspalum notatum pollen

Davies, Janet M., Mittag, Diana, Thanh D. Dang, Symons, Karen, Voskamp, Astrid, Rolland, Jennifer M. and O'Hehir, Robyn E. (2008) Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahai grass Paspalum notatum pollen. Molecular Immunology, 46 2: 286-293. doi:10.1016/j.molimm.2008.08.267


Author Davies, Janet M.
Mittag, Diana
Thanh D. Dang
Symons, Karen
Voskamp, Astrid
Rolland, Jennifer M.
O'Hehir, Robyn E.
Title Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahai grass Paspalum notatum pollen
Formatted title
Molecular cloning, expression and immunological characterisation of Pas n 1, the major allergen of Bahai grass Paspalum notatum pollen
Journal name Molecular Immunology   Check publisher's open access policy
ISSN 0161-5890
Publication date 2008-12
Sub-type Article (original research)
DOI 10.1016/j.molimm.2008.08.267
Volume 46
Issue 2
Start page 286
End page 293
Total pages 8
Place of publication Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Subject 11 Medical and Health Sciences
1107 Immunology
Formatted abstract
Bahia grass, Paspalum notatum, is a clinically important subtropical grass with a prolonged pollination season from spring to autumn. We aimed to clone and characterise the major Bahia grass pollen allergen, Pas n 1. Grass pollen-allergic patients presenting to a tertiary hospital allergy clinic were tested for IgE reactivity with Bahia grass pollen extract by skin prick testing, ImmunoCAP, ELISA and immunoblotting. Using primers deduced from the N-terminal peptide sequence of a group 1 allergen of Bahia grass pollen extract separated by two-dimensional gel electrophoresis, the complete Pas n 1 cDNA was obtained by rapid amplification of cDNA ends and cloned. Biological relevance of recombinant Pas n 1 expressed in Escherichia coli was assessed by serum IgE reactivity and basophil activation. Twenty-nine of 34 (85%) consecutive patients presenting with grass pollen allergy were skin prick test positive to Bahia grass pollen. The Pas n 1 cDNA has sequence homology with the β-expansin 1 glycoprotein family and is more closely related to the maize pollen group 1 allergen (85% identity) than to ryegrass Lol p 1 or Timothy grass Phl p 1 (64 and 66% identity, respectively). rPas n 1 reacted with serum IgE in 47 of 55 (85%) Bahia grass pollen-allergic patients, activated basophils and inhibited serum IgE reactivity with the 29 kDa band of Bahia grass pollen extract. In conclusion the cDNA for the major group 1 allergen of the subtropical Bahia grass pollen, Pas n 1, was identified and cloned. rPas n 1 is immunologically active and is a valuable reagent for diagnosis and specific immunotherapy of grass pollen allergy.


Keyword Group 1 allergen
Pas n 1
Allergic rhinitis
Bahia grass pollen
Paspalum notatu
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Thu, 02 Apr 2009, 18:19:12 EST by Maree Knight on behalf of School of Medicine