Assembly of multilayer PSS/PAH membrane on coherent alginate/PLO microcapsule for long-term graft transplantation

Leung, Andy, Trau, Matt and Nielsen, Lars Keld (2009) Assembly of multilayer PSS/PAH membrane on coherent alginate/PLO microcapsule for long-term graft transplantation. Journal of Biomedical Materials Research Part A, 88A 1: 226-237.


Author Leung, Andy
Trau, Matt
Nielsen, Lars Keld
Title Assembly of multilayer PSS/PAH membrane on coherent alginate/PLO microcapsule for long-term graft transplantation
Journal name Journal of Biomedical Materials Research Part A  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2009-01
Sub-type Article
DOI 10.1002/jbm.a.31891
Volume number 88A
Issue number 1
ISSN 1549-3296
Start page 226
End page 237
Total pages 12
Place of publication Hoboken, N. J., United States
Publisher Wiley-Liss
Collection year 2009
Language eng
Subject C1
039999 Chemical Sciences not elsewhere classified
970103 Expanding Knowledge in the Chemical Sciences
Abstract Conventional alginate/poly-L-ornithine (AP) membranes used to immunoisolate foreign tissue transplants fail in long-term transplantations of immortal cell lines. We have developed a novel layer-by-layer (LbL) membrane using polystyrene sulfonate and polyallylamine hydrochloride (PSS/PAH) on top of the coherent AP membrane. Assembly of the LbL membrane was followed by electrophoresis, and the surface morphologies and structure were characterized and examined by cryo-scanning electron microscope and transmission electron microscopy. Unlike the standard AP membrane, the LbL membrane withstood the internal pressure generated by continuous cell proliferation of microencapsulated HEK-293 and Min-6 cells. The new membrane did not affect insulin secretion or diffusion by Min-6 cells. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Keyword microencapsulation
continuous cell line
integrity
layer-by-layer membrane
insulin secretion
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Mon, 30 Mar 2009, 12:30:02 EST by Mrs Jennifer Brown on behalf of Aust Institute for Bioengineering & Nanotechnology