A novel gel-based method for self collection and ambient temperature postal transport of urine for PCR detection of Chlamydia trachomatis.

Bialasiewicz, S., Whiley, D. M., Buhrer-Skinner, M., Bautista, C., Barker, K., Aitken, S., Gordon, R., Muller, R., Lambert, S. B., Debattista, J., Nissen, M. D. and Sloots, T. P. (2009) A novel gel-based method for self collection and ambient temperature postal transport of urine for PCR detection of Chlamydia trachomatis.. Sexually Transmitted Infections, 85 2: 102-105.


Author Bialasiewicz, S.
Whiley, D. M.
Buhrer-Skinner, M.
Bautista, C.
Barker, K.
Aitken, S.
Gordon, R.
Muller, R.
Lambert, S. B.
Debattista, J.
Nissen, M. D.
Sloots, T. P.
Title A novel gel-based method for self collection and ambient temperature postal transport of urine for PCR detection of Chlamydia trachomatis.
Journal name Sexually Transmitted Infections   Check publisher's open access policy
ISSN 1368-4973
Publication date 2009-04
Year available 2008
Sub-type Article (original research)
DOI 10.1136/sti.2008.032607
Volume 85
Issue 2
Start page 102
End page 105
Total pages 4
Editor Jackie Cassell
Place of publication London, England
Publisher BMJ Publishing Group
Language eng
Subject C1
060502 Infectious Agents
060503 Microbial Genetics
060506 Virology
110309 Infectious Diseases
Abstract Objectives: The aim of this study was to develop a novel urine transport method to be used in self-collection-based screening for Chlamydia trachomatis. The method needed to be suitable for C trachomatis PCR detection, be economical and suitable for transport by standard envelope mailing. Methods: An anhydrous gel composed of super-absorbent polymer and buffering agent was used to desiccate urine into a dry granulous state, which could subsequently be reconstituted upon arrival at a laboratory. DNA was then extracted from the reconstituted solution using the Roche MagNA Pure protocol for the detection of C trachomatis by PCR. Collections of urine specimens from three populations with widely differing chlamydia prevalence (100%,n = 56; 47%, n = 70; 3%, n = 97) were used. We determined the gel method’s impact on C trachomatis PCR sensitivity and specificity using neat and gel-processed urine specimens. An equine herpes virus PCR was used to test for assay inhibition. Results: Overall, the sensitivity of the gel-based method ranged from 94.6–100% compared with neat urine, with a specificity of 100%. No PCR inhibition or decrease in analytical sensitivity was observed using the gel-processed extracts. Conclusions: The gel-based method was found to be suitable for the detection of C trachomatis by PCR. In addition, its ease of use, effectiveness at ambient temperature and low cost makes it well-suited for self-collection kits used in population-based C trachomatis screening, particularly for geographically and socially isolated individuals.
Formatted abstract Objectives: The aim of this study was to develop a novel urine transport method to be used in self-collection-based screening for Chlamydia trachomatis. The method needed to be suitable for C trachomatis PCR detection, be economical and suitable for transport by standard envelope mailing.

Methods: An anhydrous gel composed of super-absorbent polymer and buffering agent was used to desiccate urine into a dry granulous state, which could subsequently be reconstituted upon arrival at a laboratory. DNA was then extracted from the reconstituted solution using the Roche MagNA Pure protocol for the detection of C trachomatis by PCR. Collections of urine specimens from three populations with widely differing chlamydia prevalence (100%,n = 56; 47%, n = 70; 3%, n = 97) were used. We determined the gel method’s impact on C trachomatis PCR sensitivity and specificity using neat and gel-processed urine specimens. An equine herpes virus PCR was used to test for assay inhibition.

Results: Overall, the sensitivity of the gel-based method ranged from 94.6–100% compared with neat urine, with a specificity of 100%. No PCR inhibition or decrease in analytical sensitivity was observed using the gel-processed extracts.

Conclusions: The gel-based method was found to be suitable for the detection of C trachomatis by PCR. In addition, its ease of use, effectiveness at ambient temperature and low cost makes it well-suited for self-collection kits used in population-based C trachomatis screening, particularly for geographically and socially isolated individuals.
Keyword Chlamydia trachomatis
Postal Survey
PCR
Self-collection kits
Urine
Neisseria-gonorrhoeae
Anhydrous gel
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Published Online First 12 November 2008

 
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Created: Wed, 25 Mar 2009, 13:55:28 EST by Lesley Arnicar on behalf of Clinical Medical Virology Centre