Osterix induces osteogenic gene expression but not differentiation in primary human fetal mesenchymal stem cells

Kurata, Hitoshi, Guillot, Pascale V., Chan, Jerry and Fisk, Nicholas M. (2007) Osterix induces osteogenic gene expression but not differentiation in primary human fetal mesenchymal stem cells. Tissue Engineering, 13 7: 1513-1523. doi:10.1089/ten.2006.0374


Author Kurata, Hitoshi
Guillot, Pascale V.
Chan, Jerry
Fisk, Nicholas M.
Title Osterix induces osteogenic gene expression but not differentiation in primary human fetal mesenchymal stem cells
Journal name Tissue Engineering   Check publisher's open access policy
ISSN 1076-3279
Publication date 2007-07
Year available 2007
Sub-type Article (original research)
DOI 10.1089/ten.2006.0374
Volume 13
Issue 7
Start page 1513
End page 1523
Total pages 11
Place of publication Larchmont, NY, U.S.A.
Publisher Mary Ann Liebert
Language eng
Subject 111401 Foetal Development and Medicine
111402 Obstetrics and Gynaecology
1114 Paediatrics and Reproductive Medicine
Formatted abstract
The transcription factor osterix (Osx) is a key regulator of osteoblast differentiation and induces bone formation in embryonic but not adult stem cells. We investigated the effect of up-regulating Osx on an intermediate stem cell type, first trimester fetal mesenchymal stem cells (MSCs), which are more expandable than adult MSCs. Human fetal (hf ) MSCs were transduced with a lentiviral vector encoding human Osx. In undifferentiating MSCs cultures, forced expression of Osx stimulated osteopontin and alkaline phosphatase expression. However, Osx did not up-regulate osteocalcin, a late marker of osteoblast differentiation or result in extracellular calcium crystals, indicating that Osx does not directly mediate terminal differentiation in primary hfMSCs. To understand the downstream effects of Osx expression in primary hfMSCs, we next investigated the regulatory relationship between Osx, and the transcription factors Dlx5, Runx2, and Msx2. Osx induced Dlx5 but did not affect Runx2 and Msx2, whereas stealth ribonucleic acid interference of Osx inhibited Dlx5 without affecting expression of Runx2 and Msx2. In conclusion, Osx regulates osteogenic gene expression in hfMSCs but is insufficient to induce terminal osteogenic differentiation.
© Mary Ann Liebert, Inc.
Keyword Transcription factor osterix
Osteoblast differentiation
Embryonic stem cells
Fetal mesenchymal stem cells
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
 
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Created: Mon, 23 Mar 2009, 14:48:28 EST by Mary-Anne Marrington on behalf of Faculty Of Health Sciences