Efforts to capture high amylose in rice

Butardo, Vito Jr. M., Fitzgerald, Melissa A., Rahman, Sadequr and Gidley, Michael J. (2008). Efforts to capture high amylose in rice. In: Cereal Foods World: Diversity of Grains. AACC International Annual Meeting 2008, Hawaii Convention Centre, Honolulu, Hawaii, (A15-A15). 21-24 September, 2008.

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CFW-53-4-suppl.aacc2008.pdf Cereal Foods World Supplement Vol 53 No 4 Page A15 application/pdf 1.29MB 856
aacc2008programbook.pdf AACC 2008 Program Book Page 29 application/pdf 14.43MB 1636
Author Butardo, Vito Jr. M.
Fitzgerald, Melissa A.
Rahman, Sadequr
Gidley, Michael J.
Title of paper Efforts to capture high amylose in rice
Formatted title

Conference name AACC International Annual Meeting 2008
Conference location Hawaii Convention Centre, Honolulu, Hawaii
Conference dates 21-24 September, 2008
Proceedings title Cereal Foods World: Diversity of Grains   Check publisher's open access policy
Place of Publication St. Paul, Minn., U.S.A.
Publisher American Association of Cereal Chemists
Publication Year 2008
Year available 2008
Sub-type Other
Open Access Status
ISSN 0146-6283
Volume 53
Issue 4
Start page A15
End page A15
Total pages 1
Language eng
Abstract/Summary Screening of wild and cultivated rice in IRRI germplasm collection revealed that majority have intermediate apparent amylose content. It appears that ancient farmers selected rice based on texture of the lower amylose varieties, considering that the majority of rice consumers today prefer intermediate to soft-textured rice. Furthermore, 30% seems to be the natural upper natural limit of amylose levels in wild-type rice. If this is the case, the rich biodiversity of rice has been subjected to the bottleneck of domestication to select for grains that have superior cooking and eating but not nutritional or satiating qualities considering that the majority of rice consumers today eat rice three times a day. On the other hand, the amylose content of available rice mutants with deficient SBEIIb or an over-expressed GBSSI also revealed amylose levels of around 35% which is significantly lower by comparison with other high amylose cereals, whose amylose content ranges from 70–90%. Hence, to produce the high amylose phenotype in rice, one might need to target different sets of enzymes or regulatory pathways. Since increasing the amylose levels in rice might mean a concomitant increase in its resistant starch content and in its levels of satiety, and a decrease in its glycemic response, developing high amylose rice by biotechnology is imperative. This type of rice will be important not only in addressing the growing obesity epidemic which now also affects the developing countries but also as a basis of novel degradable biopolymers and for further elucidating the mechanisms of starch synthesis in the cereal endosperm. In this paper, we also present the status of our research project which aims to silence the expression of SBEIIa, SBEIIb and SSIIa singly or in combination using microRNA and RNAi silencing technologies with the aim of increasing the amylose levels in rice beyond its natural limits.
Subjects 0703 Crop and Pasture Production
Keyword Rice
amylose content
wild rice
Rna Interference (rnai)
Apparent High Amylose
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: School of Agriculture and Food Sciences
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Created: Fri, 20 Mar 2009, 13:59:23 EST by Mr Vito Butardo on behalf of Faculty Of Nat Resources, Agric & Veterinary Sc