Rapid and improved reconstitution of bacterial mechanosensitive ion channel proteins MscS and MscL into liposomes using a modified sucrose method

Battle, Andrew R., Petrov, Evgeny, Pal, Prithwish and Martinac, Boris (2009) Rapid and improved reconstitution of bacterial mechanosensitive ion channel proteins MscS and MscL into liposomes using a modified sucrose method. FEBS Letters, 583 2: 407-412. doi:10.1016/j.febslet.2008.12.033


Author Battle, Andrew R.
Petrov, Evgeny
Pal, Prithwish
Martinac, Boris
Title Rapid and improved reconstitution of bacterial mechanosensitive ion channel proteins MscS and MscL into liposomes using a modified sucrose method
Journal name FEBS Letters   Check publisher's open access policy
ISSN 0014-5793
Publication date 2009
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.febslet.2008.12.033
Volume 583
Issue 2
Start page 407
End page 412
Total pages 6
Editor F. Wieland
Place of publication Netherlands
Publisher Elsevier BV
Collection year 2009
Language eng
Subject 060110 Receptors and Membrane Biology
970106 Expanding Knowledge in the Biological Sciences
C1
Abstract The bacterial mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance have functionally been reconstituted into giant unilamellar liposomes (GUVs) using an improved reconstitution method in the presence of sucrose. This method gives significant time savings (preparation times as little as 6h) compared to the classical method of protein reconstitution which uses a dehydration/rehydration (D/R) procedure (minimum 2 days preparation time). Moreover, it represents the first highly reproducible method for functional reconstitution of MscS as well as MscS/MscL co-reconstitution. This novel procedure has the potential to be used for studies of other ion channels by liposome reconstitution.
Keyword Patch clamp
Confocal microscopy
Fluorescence
Lipid
Sugar
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biomedical Sciences Publications
 
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Created: Wed, 18 Mar 2009, 12:12:28 EST