The pilin O-glycosylation pathway of pathogenic Neisseria is a general system that glycosylates AniA, an outer membrane nitrite reductase

Ku, S.C., Schulz, B.L., Power, P.M. and Jennings, M.P. (2009) The pilin O-glycosylation pathway of pathogenic Neisseria is a general system that glycosylates AniA, an outer membrane nitrite reductase. Biochemical and Biophysical Research Communications, 378 1: 84-89. doi:10.1016/j.bbrc.2008.11.025


Author Ku, S.C.
Schulz, B.L.
Power, P.M.
Jennings, M.P.
Title The pilin O-glycosylation pathway of pathogenic Neisseria is a general system that glycosylates AniA, an outer membrane nitrite reductase
Formatted title
The pilin O-glycosylation pathway of pathogenic Neisseria is a general system that glycosylates AniA, an outer membrane nitrite reductase
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2009-01-02
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2008.11.025
Volume 378
Issue 1
Start page 84
End page 89
Total pages 6
Place of publication New York, United States
Publisher Academic Press
Collection year 2009
Language eng
Subject C1
060503 Microbial Genetics
920109 Infectious Diseases
Abstract O-Glycosylation is emerging as a common posttranslational modification of surface exposed proteins in bacterial mucosal pathogens. In pathogenic Neisseria an O-glycosylation pathway modifies a single abundant protein, pilin, the subunit protein that forms pili. Here, we identify an additional outer membrane glycoprotein in pathogenic Neisseria, the nitrite reductase AniA, that is glycosylated in its C-terminal repeat region by the pilin glycosylation pathway. To our knowledge, this is the first report of a general O-glycosylation pathway in a prokaryote. We also show that AniA displays polymorphisms in residues that map to the surface of the protein. A frame-shift mutation abolishes AniA expression in 34% of Neisseria meningitidis strains surveyed, however, all Neisseria gonorrhoeae strains examined are predicted to express AniA, implying a Crucial role for AniA in gonococcal biology. (C) 2008 Elsevier Inc. All rights reserved.
Keyword Glycosylation
O-Glycosulation
Neisseria meningitis
Neisseria gonorrhoeae
AniA
Nitrite reductase
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Received 24 October 2008. Available online 14 November 2008.

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 17 Mar 2009, 11:30:13 EST by Jennifer Falknau on behalf of School of Chemistry & Molecular Biosciences