The essential and non-essential genes of Bovine herpesvirus 1

Robinson, Karl E., Meers, Joanne, Gravel, Jennifer L., McCarthy, Fiona M. and Mahony, Timothy J. (2008) The essential and non-essential genes of Bovine herpesvirus 1. Journal of General Virology, 89 11: 2851-2863. doi:10.1099/vir.0.2008/002501-0

Author Robinson, Karl E.
Meers, Joanne
Gravel, Jennifer L.
McCarthy, Fiona M.
Mahony, Timothy J.
Title The essential and non-essential genes of Bovine herpesvirus 1
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
Publication date 2008-11
Sub-type Article (original research)
DOI 10.1099/vir.0.2008/002501-0
Open Access Status
Volume 89
Issue 11
Start page 2851
End page 2863
Total pages 13
Place of publication Reading, England
Publisher Society for General Microbiology
Collection year 2009
Language eng
Subject C1
070712 Veterinary Virology
970107 Expanding Knowledge in the Agricultural and Veterinary Sciences
Abstract Bovine herpesvirus 1 (BoHV-1) is an economically important pathogen of cattle associated with respiratory and reproductive disease. To further develop BoHV-1 as a vaccine vector, a study was conducted to identify the essential and non-essential genes required for in vitro viability. Random-insertion mutagenesis utilizing a Tn5 transposition system and targeted gene deletion were employed to construct gene disruption and gene deletion libraries, respectively, of an infectious clone of BoHV-1. Transposon insertion position and confirmation of gene deletion were determined by direct sequencing. The essential or non-essential requirement of either transposed or deleted open reading frames (ORFs) was assessed by transfection of respective BoHV-1 DNA into host cells. Of the 73 recognized ORFs encoded by the BoHV-1 genome, 33 were determined to be essential and 36 to be non-essential for virus viability in cell culture; determining the requirement of the two dual copy ORFs was inconclusive. The majority of ORFs were shown to conform to the in vitro requirements of BoHV-1 homologues encoded by human herpesvirus 1 (HHV-1). However, ORFs encoding glycoprotein K (UL53), regulatory, membrane, tegument and capsid proteins (UL54, UL49.5, UL49, UL35, UL20, UL16 and UL7) were shown to differ in requirement when compared to HHV-1-encoded homologues.
Artificial Chromosome
Q-Index Code C1
Q-Index Status Confirmed Code

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Created: Thu, 05 Mar 2009, 14:40:42 EST by Narelle Poole on behalf of School of Veterinary Science