Toll-like receptor 2 ligands inhibit Th2 responses to mite allergen

Taylor, Rebecca C., Richmond, Peter and Upham, John W. (2006) Toll-like receptor 2 ligands inhibit Th2 responses to mite allergen. Journal of Allergy and Clinical Immunology, 117 5: 1148-1154. doi:10.1016/j.jaci.2006.02.014

Author Taylor, Rebecca C.
Richmond, Peter
Upham, John W.
Title Toll-like receptor 2 ligands inhibit Th2 responses to mite allergen
Formatted title
Toll-like receptor 2 ligands inhibit TH2 responses to mite allergen
Journal name Journal of Allergy and Clinical Immunology   Check publisher's open access policy
ISSN 0091-6749
Publication date 2006-05
Sub-type Article (original research)
DOI 10.1016/j.jaci.2006.02.014
Volume 117
Issue 5
Start page 1148
End page 1154
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Mosby
Language eng
Subject 110701 Allergy
110203 Respiratory Diseases
Formatted abstract
There is intense interest in the interaction between microbial compounds and allergy. Although Toll-like receptor (TLR)–2 ligands derived from Gram-positive bacteria alter allergic sensitization in animal models, it is not clear what effect TLR2 ligands have on allergen-specific T-cell memory in human beings.

To determine whether in vitro exposure to TLR2 ligands modifies the immune response to house dust mite allergen (HDM).

Blood mononuclear cells were obtained from individuals both allergic (n = 23) and not allergic (n = 22) to HDM, and stimulated with HDM in the presence or absence of TLR2 ligands.

In subjects allergic to HDM, IL-5 and IL-13 responses to HDM were inhibited by heat-killed Staphylococcus aureus, staphylococcal lipoteichoic acid, and the synthetic lipoprotein Pam3CSK4 (P < .005; all stimuli). Although the whole staphylococcal bacteria increased IFN-γ responses, the purified TLR2 ligands lipoteichoic acid and Pam3CSK4 inhibited HDM-specific IFN-γ synthesis. In contrast, TLR2 ligands had minimal effects on responses to HDM in subjects without allergy. TLR2 ligands induced upregulation of HLA-DR expression but did not inhibit antigen uptake or processing by antigen-presenting cells.


Toll-like receptor 2 ligands inhibit allergen-specific TH2 responses in sensitized individuals. This effect appears to be mediated by the actions of TLR2 ligands on antigen-presenting cells, and at least for the purified TLR2 ligands does not involve the induction of a strong TH1 immune response.

Clinical implications
These findings provide an impetus for further preclinical studies examining the potential use of TLR2 ligands in allergic disease.
Keyword Atopy
Toll-like receptors
Innate immunity
TH2 cytokines
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
ERA 2012 Admin Only
School of Medicine Publications
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Created: Wed, 04 Mar 2009, 10:59:49 EST by Laura McTaggart on behalf of Medicine - Princess Alexandra Hospital