HLA-DR expression on neonatal monocytes is associated with allergen-specific immune responses

Upham, John W., Holt, Patrick G., Taylor, Angie, Thornton, Catherine A. and Prescott, Susan L. (2004) HLA-DR expression on neonatal monocytes is associated with allergen-specific immune responses. Journal of Allergy and Clinical Immunology, 114 5: 1202-1208. doi:10.1016/j.jaci.2004.06.051

Author Upham, John W.
Holt, Patrick G.
Taylor, Angie
Thornton, Catherine A.
Prescott, Susan L.
Title HLA-DR expression on neonatal monocytes is associated with allergen-specific immune responses
Journal name Journal of Allergy and Clinical Immunology   Check publisher's open access policy
ISSN 0091-6749
Publication date 2004-11
Sub-type Article (original research)
DOI 10.1016/j.jaci.2004.06.051
Volume 114
Issue 5
Start page 1202
End page 1208
Total pages 7
Place of publication St Louis, MO, USA
Publisher Mosby
Language eng
Subject 110701 Allergy
110704 Cellular Immunology
Abstract Background The specific mechanisms regulating priming of T-cell immunity to common allergens during early childhood remain to be elucidated, though increasing evidence indicates that antigen-presenting cell function is impaired in childhood. Objective Examine the relationship between HLA-DR expression on monocytes and B cells, allergen-specific T-cell responses at birth, and clinical outcomes at 2 years of age. Methods Blood mononuclear cells were obtained from 36 healthy neonates who were followed up clinically to the age of 2 years. Expression of HLA-DR by monocytes and B cells was determined at baseline and after in vitro exposure to IFN-γ, a cytokine that is known to upregulate the expression of HLA-DR. Mononuclear cells were stimulated with endotoxin or a panel of inhalant and food allergens, and cytokine responses and lymphoproliferation were determined after 1 and 5 days, respectively. Results The magnitude of HLA-DR upregulation on IFN-γ–stimulated cord blood CD14+ monocytes was consistently correlated with allergen-induced, but not mitogen-induced, lymphoproliferation at birth. HLA-DR upregulation on monocytes was also positively associated with endotoxin-induced IL-12 p70 synthesis (τ = 0.46; P < .001) but inversely related to mite- and ovalbumin-induced IL-13 synthesis (P = .0006 and P < .003, respectively). HLA-DR expression on unstimulated cord blood monocytes was inversely associated with symptoms of atopic disease at the 2-year follow-up (P = .015). In contrast, HLA-DR expression on B cells was not associated with these parameters of immune function. Conclusions These findings suggest that the maturity of neonatal monocytes and their responsiveness to external stimuli are linked to differing patterns of immune reactivity at birth and to the risk of allergic symptoms in early childhood.
Keyword Neonatal immune function
B cells
Antigen-presenting cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Medicine Publications
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Created: Wed, 04 Mar 2009, 10:20:27 EST by Ms Sarada Rao on behalf of School of Medicine